Abstract
Objective: The present research intends to isolate Nanjing local strains of human herpesvirus 7 (HHV-7). Methods: Saliva were sampled from health individuals and from children with kidney disease. After treatment with antibiotics and filtering, the samples were inoculated to the umbilical cord blood mononuclear cells (CBMCs) stimulated by phytohemagglutinin. When the infected cells presented the typical ballooning and polykaryotic cytopathic effects (CPE) , transmission electron microscopy of cells was performed. DNA was extracted and polymerase chain reaction was carried out using the primers specific for HHV-7. The PCR products were also sequenced. Results: Four strains were isolated from the seven saliva specimens. Electronic microscopy showed the presence of virus-like particles with the characteristic features of herpesviruses. The expected PCR product was amplified from both isolates. The specificity of the reaction was confirmed by DNA sequence and 186bp fragments revealed an identity with HHV-7 RK and JI of 100%. Conclusion: The isolated virus was HHV-7.
| Original language | English |
|---|---|
| Pages (from-to) | 521-524 |
| Number of pages | 4 |
| Journal | Chinese Journal of Microbiology and Immunology |
| Volume | 20 |
| Issue number | 6 |
| State | Published - Nov 2000 |
Keywords
- DNA sequence
- Human herpesvirus 7
- PCR
- Virus isolation