Isolation of human basophils by flow microfluorometry

Gary J. Weil; William M. Leiserson; Thomas M. Chused

doi:10.1016/0022-1759(83)90363-0

Isolation of human basophils by flow microfluorometry

Gary J. Weil, William M. Leiserson, Thomas M. Chused

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.

Original language	English
Pages (from-to)	359-363
Number of pages	5
Journal	Journal of Immunological Methods
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(83)90363-0
State	Published - Mar 25 1983

Keywords

basophils
cell separation
flow microfluorometry

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10.1016/0022-1759(83)90363-0

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@article{83badd4602e2457ca9093dd1e680cae8,

title = "Isolation of human basophils by flow microfluorometry",

abstract = "Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.",

keywords = "basophils, cell separation, flow microfluorometry",

author = "Weil, {Gary J.} and Leiserson, {William M.} and Chused, {Thomas M.}",

year = "1983",

month = mar,

day = "25",

doi = "10.1016/0022-1759(83)90363-0",

language = "English",

volume = "58",

pages = "359--363",

journal = "Journal of Immunological Methods",

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T1 - Isolation of human basophils by flow microfluorometry

AU - Weil, Gary J.

AU - Leiserson, William M.

AU - Chused, Thomas M.

PY - 1983/3/25

Y1 - 1983/3/25

N2 - Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.

AB - Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.

KW - basophils

KW - cell separation

KW - flow microfluorometry

UR - http://www.scopus.com/inward/record.url?scp=0020628799&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(83)90363-0

DO - 10.1016/0022-1759(83)90363-0

M3 - Article

C2 - 6833772

AN - SCOPUS:0020628799

SN - 0022-1759

VL - 58

SP - 359

EP - 363

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3

ER -

Isolation of human basophils by flow microfluorometry

Abstract

Keywords

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