Isolation of human basophils by flow microfluorometry

Gary J. Weil, William M. Leiserson, Thomas M. Chused

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalJournal of Immunological Methods
Volume58
Issue number3
DOIs
StatePublished - Mar 25 1983
Externally publishedYes

Keywords

  • basophils
  • cell separation
  • flow microfluorometry

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