TY - JOUR
T1 - Isolation of fetal nucleated red blood cells from maternal blood in normal and aneuploid pregnancies
AU - Prieto, Belén
AU - Cándenas, Mercedes
AU - Venta, Rafael
AU - Ladenson, Jack H.
AU - Álvarez, Francisco V.
N1 - Funding Information:
This project was supported by the Fondo de Investigación Sanitaria (98/1530), Fundación para el Fomento en Asturias de la Investigación Científica y Aplicada (PB-SAL99-02) and Fun-dación Inocente Inocente. We thank all the women who participated in this study, and all of the physicians who collaborated by sending us maternal blood samples. We also thank Rosalía García for her excellent technical work, as well as Jitka Olander for the critical comments on the manuscript. The antibodies used in this paper are under option for exclusive license at this time through the Washington University Center of Technology Management.
PY - 2002
Y1 - 2002
N2 - Fetal nucleated red blood cells (NRBC) have been widely reported in maternal blood during pregnancy. However, there is no consensus with regard to their presence in all pregnancies. Therefore, the usefulness of developing a feel NRBC-based nonin,asi,e method suitable for clinical prenatal diagnosis remains uncertain. Fluorescence in situ hybridization (FISH) method was used to evaluate the ability of one of our own monoclonal antibodies (mAb). 2B7.4, to isolate feel NRBC from maternal blood by magnetic activated cell sorting (MACS). Our mAb was able to isolate from 25 to 822 NRBC from all of the 45 maternal blood samples included in this study. A correct diagnosis was achieved in 21 out of 24 pregnancies carrying trisomic, fetuses (87.5%), with a fetal/maternal NRBC frequency of 8.4%. In contrast, a significantly lower percentage of fetal NRBC (0.2%) was observed in 22% of pregnancies carrying a chromosomally normal male fetus, that were correctly predicted. In conclusion, using 2B7.4 mAb we succeeded in isolating NRBC from the maternal blood samples, but most of the isolated cells were maternal in origin. Nevertheless, a higher number of fetal NRBC was found in the peripheral blood of pregnant women carrying aneuplold fetuses, which could allow development of a screaning method for prenatal diagnosis of fetal aneuploidies.
AB - Fetal nucleated red blood cells (NRBC) have been widely reported in maternal blood during pregnancy. However, there is no consensus with regard to their presence in all pregnancies. Therefore, the usefulness of developing a feel NRBC-based nonin,asi,e method suitable for clinical prenatal diagnosis remains uncertain. Fluorescence in situ hybridization (FISH) method was used to evaluate the ability of one of our own monoclonal antibodies (mAb). 2B7.4, to isolate feel NRBC from maternal blood by magnetic activated cell sorting (MACS). Our mAb was able to isolate from 25 to 822 NRBC from all of the 45 maternal blood samples included in this study. A correct diagnosis was achieved in 21 out of 24 pregnancies carrying trisomic, fetuses (87.5%), with a fetal/maternal NRBC frequency of 8.4%. In contrast, a significantly lower percentage of fetal NRBC (0.2%) was observed in 22% of pregnancies carrying a chromosomally normal male fetus, that were correctly predicted. In conclusion, using 2B7.4 mAb we succeeded in isolating NRBC from the maternal blood samples, but most of the isolated cells were maternal in origin. Nevertheless, a higher number of fetal NRBC was found in the peripheral blood of pregnant women carrying aneuplold fetuses, which could allow development of a screaning method for prenatal diagnosis of fetal aneuploidies.
KW - FISH
KW - Fetal erythroblasts
KW - Magnetic activated cell sorting (MACS)
KW - Prenatal diagnosis
UR - http://www.scopus.com/inward/record.url?scp=0036367002&partnerID=8YFLogxK
U2 - 10.1515/CCLM.2002.114
DO - 10.1515/CCLM.2002.114
M3 - Article
C2 - 12241011
AN - SCOPUS:0036367002
SN - 1434-6621
VL - 40
SP - 667
EP - 672
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
IS - 7
ER -