Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)

Michael Carr; Akira Kawaguchi; Michihito Sasaki; Gabriel Gonzalez; Kimihito Ito; Yuka Thomas; Bernard M. Hang’ombe; Aaron S. Mweene; Guoyan Zhao; David Wang; Yasuko Orba; Akihiro Ishii; Hirofumi Sawa

doi:10.1007/s00705-016-3129-8

Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)

Michael Carr, Akira Kawaguchi, Michihito Sasaki, Gabriel Gonzalez, Kimihito Ito, Yuka Thomas, Bernard M. Hang’ombe, Aaron S. Mweene, Guoyan Zhao, David Wang, Yasuko Orba, Akihiro Ishii, Hirofumi Sawa

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8 Scopus citations

Abstract

To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4⁺ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.

Original language	English
Pages (from-to)	543-548
Number of pages	6
Journal	Archives of Virology
Volume	162
Issue number	2
DOIs	https://doi.org/10.1007/s00705-016-3129-8
State	Published - Feb 1 2017

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title = "Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)",

abstract = "To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.",

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AU - Carr, Michael

AU - Kawaguchi, Akira

AU - Sasaki, Michihito

AU - Gonzalez, Gabriel

AU - Ito, Kimihito

AU - Thomas, Yuka

AU - Hang’ombe, Bernard M.

AU - Mweene, Aaron S.

AU - Zhao, Guoyan

AU - Wang, David

AU - Orba, Yasuko

AU - Ishii, Akihiro

AU - Sawa, Hirofumi

PY - 2017/2/1

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AB - To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.

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Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)

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