TY - JOUR
T1 - Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)
AU - Carr, Michael
AU - Kawaguchi, Akira
AU - Sasaki, Michihito
AU - Gonzalez, Gabriel
AU - Ito, Kimihito
AU - Thomas, Yuka
AU - Hang’ombe, Bernard M.
AU - Mweene, Aaron S.
AU - Zhao, Guoyan
AU - Wang, David
AU - Orba, Yasuko
AU - Ishii, Akihiro
AU - Sawa, Hirofumi
N1 - Funding Information:
We would like to thank the Department of National Parks and Wildlife of the Ministry of Tourism and Arts (formerly ZAWA) for assistance with sample collection in Zambia. This study was supported in part by grants for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Science, Sports and Technology (MEXT) of Japan (16H06429, 16H06431, 16K21723); grants from the Ministry of Education, Culture, Sports, Science and Technology; the Ministry of Health, Labour and Welfare, Japan (MEXT)/JSPS KAKENHI (16H05805); and the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), MEXT Japan.
Publisher Copyright:
© 2016, Springer-Verlag Wien.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
AB - To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
UR - http://www.scopus.com/inward/record.url?scp=84994097213&partnerID=8YFLogxK
U2 - 10.1007/s00705-016-3129-8
DO - 10.1007/s00705-016-3129-8
M3 - Article
C2 - 27804019
AN - SCOPUS:84994097213
SN - 0304-8608
VL - 162
SP - 543
EP - 548
JO - Archives of Virology
JF - Archives of Virology
IS - 2
ER -