Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Margaret L. Palmer; Mary Alice Raker; Poindexter J. Kennedy; Jon W. Young; Wayne M. Barnes; Raymond L. Rodriguez; Harry F. Noller

doi:10.1007/BF00268279

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Margaret L. Palmer
, Mary Alice Raker
, Poindexter J. Kennedy
, Jon W. Young
, Wayne M. Barnes
, Raymond L. Rodriguez
, Harry F. Noller

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

Original language	English
Pages (from-to)	171-178
Number of pages	8
Journal	MGG Molecular & General Genetics
Volume	172
Issue number	2
DOIs	https://doi.org/10.1007/BF00268279
State	Published - Jan 1979

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10.1007/BF00268279

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title = "Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli",

abstract = "Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.",

author = "Palmer, \{Margaret L.\} and Raker, \{Mary Alice\} and Kennedy, \{Poindexter J.\} and Young, \{Jon W.\} and Barnes, \{Wayne M.\} and Rodriguez, \{Raymond L.\} and Noller, \{Harry F.\}",

year = "1979",

month = jan,

doi = "10.1007/BF00268279",

language = "English",

volume = "172",

pages = "171--178",

journal = "MGG Molecular \& General Genetics",

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T1 - Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

AU - Palmer, Margaret L.

AU - Raker, Mary Alice

AU - Kennedy, Poindexter J.

AU - Young, Jon W.

AU - Barnes, Wayne M.

AU - Rodriguez, Raymond L.

AU - Noller, Harry F.

PY - 1979/1

Y1 - 1979/1

N2 - Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

AB - Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

UR - https://www.scopus.com/pages/publications/0018422126

U2 - 10.1007/BF00268279

DO - 10.1007/BF00268279

M3 - Article

C2 - 384145

AN - SCOPUS:0018422126

SN - 0026-8925

VL - 172

SP - 171

EP - 178

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 2

ER -

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Abstract

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