Isolation and patch-clamp of primary adipocytes

Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith, Rajan Sah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages145-150
Number of pages6
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1566
ISSN (Print)1064-3745

Keywords

  • Adipocyte isolation
  • Culture
  • Differentiation
  • Patch-clamp
  • Perforated-patch
  • Whole-cell patch-clamp

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