Isolation and organization of genes for nitrogen fixation in Rhodopseudomonas capsulata

Peter Avtges; Robert G. Kranz; Robert Haselkorn

doi:10.1007/BF00331324

Isolation and organization of genes for nitrogen fixation in Rhodopseudomonas capsulata

Peter Avtges, Robert G. Kranz, Robert Haselkorn

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

A library of Rhodopseudomonas capsulata chromosomal DNA was constructed in the broad host range cosmid vector pLAFR1. The library was used to isolate nitrogen fixation genes by complementation of R. capsulata Nif^- mutants. Four complementing regions were localized on different cloned DNA fragments by Tn 5 and mini-Mu mutagenesis. Additional nif genes were identified by recombination of transposons from the nif cosmids into the R. capsulata chromosome resulting in the creation of new Nif^- mutations. Most of the newly cloned DNA fragments containing nif genes were found to be unlinked to any other by Southern hybridization of the cloned DNA to chromosomal DNA blots. One of the new fragments was linked to the nifHDK genes. Another cluster spanning 10-12 kilobase pairs contained a number of nif genes, possibly as many as eight.

Original language	English
Pages (from-to)	363-369
Number of pages	7
Journal	MGG Molecular & General Genetics
Volume	201
Issue number	3
DOIs	https://doi.org/10.1007/BF00331324
State	Published - Dec 1985

Access to Document

10.1007/BF00331324

Cite this

@article{06443935bef84ee89ea2d00e6bea4e58,

title = "Isolation and organization of genes for nitrogen fixation in Rhodopseudomonas capsulata",

abstract = "A library of Rhodopseudomonas capsulata chromosomal DNA was constructed in the broad host range cosmid vector pLAFR1. The library was used to isolate nitrogen fixation genes by complementation of R. capsulata Nif- mutants. Four complementing regions were localized on different cloned DNA fragments by Tn 5 and mini-Mu mutagenesis. Additional nif genes were identified by recombination of transposons from the nif cosmids into the R. capsulata chromosome resulting in the creation of new Nif- mutations. Most of the newly cloned DNA fragments containing nif genes were found to be unlinked to any other by Southern hybridization of the cloned DNA to chromosomal DNA blots. One of the new fragments was linked to the nifHDK genes. Another cluster spanning 10-12 kilobase pairs contained a number of nif genes, possibly as many as eight.",

author = "Peter Avtges and Kranz, {Robert G.} and Robert Haselkorn",

year = "1985",

month = dec,

doi = "10.1007/BF00331324",

language = "English",

volume = "201",

pages = "363--369",

journal = "MGG Molecular & General Genetics",

issn = "0026-3925",

number = "3",

}

TY - JOUR

T1 - Isolation and organization of genes for nitrogen fixation in Rhodopseudomonas capsulata

AU - Avtges, Peter

AU - Kranz, Robert G.

AU - Haselkorn, Robert

PY - 1985/12

Y1 - 1985/12

N2 - A library of Rhodopseudomonas capsulata chromosomal DNA was constructed in the broad host range cosmid vector pLAFR1. The library was used to isolate nitrogen fixation genes by complementation of R. capsulata Nif- mutants. Four complementing regions were localized on different cloned DNA fragments by Tn 5 and mini-Mu mutagenesis. Additional nif genes were identified by recombination of transposons from the nif cosmids into the R. capsulata chromosome resulting in the creation of new Nif- mutations. Most of the newly cloned DNA fragments containing nif genes were found to be unlinked to any other by Southern hybridization of the cloned DNA to chromosomal DNA blots. One of the new fragments was linked to the nifHDK genes. Another cluster spanning 10-12 kilobase pairs contained a number of nif genes, possibly as many as eight.

AB - A library of Rhodopseudomonas capsulata chromosomal DNA was constructed in the broad host range cosmid vector pLAFR1. The library was used to isolate nitrogen fixation genes by complementation of R. capsulata Nif- mutants. Four complementing regions were localized on different cloned DNA fragments by Tn 5 and mini-Mu mutagenesis. Additional nif genes were identified by recombination of transposons from the nif cosmids into the R. capsulata chromosome resulting in the creation of new Nif- mutations. Most of the newly cloned DNA fragments containing nif genes were found to be unlinked to any other by Southern hybridization of the cloned DNA to chromosomal DNA blots. One of the new fragments was linked to the nifHDK genes. Another cluster spanning 10-12 kilobase pairs contained a number of nif genes, possibly as many as eight.

UR - http://www.scopus.com/inward/record.url?scp=0022412151&partnerID=8YFLogxK

U2 - 10.1007/BF00331324

DO - 10.1007/BF00331324

M3 - Article

C2 - 3003541

AN - SCOPUS:0022412151

SN - 0026-3925

VL - 201

SP - 363

EP - 369

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 3

ER -

Isolation and organization of genes for nitrogen fixation in Rhodopseudomonas capsulata

Abstract

Access to Document

Other files and links

Fingerprint

Cite this