Isolation and identification of two novel SDS-Resistant secreted chitinases from Aeromonas schubertii

Chao Lin Liu; Chia Rui Shen; Fong Fu Hsu; Jeen Kuan Chen; Pei Tzu Wu; Shang Hsin Guo; Wen Chien Lee; Feng Wei Yu; Zachary B. Mackey; John Turk; Michael L. Gross

doi:10.1002/btpr.100

Isolation and identification of two novel SDS-Resistant secreted chitinases from Aeromonas schubertii

Chao Lin Liu, Chia Rui Shen, Fong Fu Hsu, Jeen Kuan Chen, Pei Tzu Wu, Shang Hsin Guo, Wen Chien Lee, Feng Wei Yu, Zachary B. Mackey, John Turk, Michael L. Gross

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass mea-surement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCHI53 and ASCHI61 retained, respectively, 65% and 75%, of their chitinase activity in the presence of5% SDS and 100% of their activity in the presence of 10%% fi-mercaptoethanol. These results demon strate that they are SDS-resistant endochitinases and probably have a rigid structure.

Original language	English
Pages (from-to)	124-131
Number of pages	8
Journal	Biotechnology Progress
Volume	25
Issue number	1
DOIs	https://doi.org/10.1002/btpr.100
State	Published - Jan 2009

Keywords

Aeromonas schubertii
Endochitinase
Hexameric chitin oligosaccharide
In-gel chitinase assay
N-acetyl-d-glucosamine (glcnac)
Sds-resistance

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title = "Isolation and identification of two novel SDS-Resistant secreted chitinases from Aeromonas schubertii",

abstract = "Two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass mea-surement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCHI53 and ASCHI61 retained, respectively, 65% and 75%, of their chitinase activity in the presence of5% SDS and 100% of their activity in the presence of 10%% fi-mercaptoethanol. These results demon strate that they are SDS-resistant endochitinases and probably have a rigid structure.",

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author = "Liu, {Chao Lin} and Shen, {Chia Rui} and Hsu, {Fong Fu} and Chen, {Jeen Kuan} and Wu, {Pei Tzu} and Guo, {Shang Hsin} and Lee, {Wen Chien} and Yu, {Feng Wei} and Mackey, {Zachary B.} and John Turk and Gross, {Michael L.}",

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doi = "10.1002/btpr.100",

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AU - Liu, Chao Lin

AU - Shen, Chia Rui

AU - Hsu, Fong Fu

AU - Chen, Jeen Kuan

AU - Wu, Pei Tzu

AU - Guo, Shang Hsin

AU - Lee, Wen Chien

AU - Yu, Feng Wei

AU - Mackey, Zachary B.

AU - Turk, John

AU - Gross, Michael L.

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AB - Two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass mea-surement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCHI53 and ASCHI61 retained, respectively, 65% and 75%, of their chitinase activity in the presence of5% SDS and 100% of their activity in the presence of 10%% fi-mercaptoethanol. These results demon strate that they are SDS-resistant endochitinases and probably have a rigid structure.

KW - Aeromonas schubertii

KW - Endochitinase

KW - Hexameric chitin oligosaccharide

KW - In-gel chitinase assay

KW - N-acetyl-d-glucosamine (glcnac)

KW - Sds-resistance

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SP - 124

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JO - Biotechnology Progress

JF - Biotechnology Progress

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ER -

Isolation and identification of two novel SDS-Resistant secreted chitinases from Aeromonas schubertii

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