Isolation and characterization of resident macrophages from guinea pig and human intestine

Harland S. Winter; F. Sessions Cole; Linda M. Huffer; Cheryl B. Davidson; Aubrey J. Katz; Paul J. Edelson

doi:10.1016/0016-5085(83)90324-4

Isolation and characterization of resident macrophages from guinea pig and human intestine

Harland S. Winter, F. Sessions Cole, Linda M. Huffer, Cheryl B. Davidson, Aubrey J. Katz, Paul J. Edelson

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The evaluation of mucosal cellular immune function in the gastrointestinal tract has focused on properties of lymphocytes. We describe methods for use in guinea pig and human tissue that will now permit the maintenance and in vitro study of intestinal macrophages. This study characterizes the resident gastrointestinal macrophage population in the two species and shows that morphologic and ultra-structural characteristics are similar to macrophages from other tissues. Histochemically, the cells are esterase positive and peroxidase negative. They possess surface receptors for immunoglobulin G and complement, and are phagocytic via the Fc receptor. In addition, guinea pig intestinal macrophages synthesize both the second and fourth components of complement.

Original language	English
Pages (from-to)	358-363
Number of pages	6
Journal	Gastroenterology
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/0016-5085(83)90324-4
State	Published - Aug 1983

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title = "Isolation and characterization of resident macrophages from guinea pig and human intestine",

abstract = "The evaluation of mucosal cellular immune function in the gastrointestinal tract has focused on properties of lymphocytes. We describe methods for use in guinea pig and human tissue that will now permit the maintenance and in vitro study of intestinal macrophages. This study characterizes the resident gastrointestinal macrophage population in the two species and shows that morphologic and ultra-structural characteristics are similar to macrophages from other tissues. Histochemically, the cells are esterase positive and peroxidase negative. They possess surface receptors for immunoglobulin G and complement, and are phagocytic via the Fc receptor. In addition, guinea pig intestinal macrophages synthesize both the second and fourth components of complement.",

author = "Winter, {Harland S.} and Cole, {F. Sessions} and Huffer, {Linda M.} and Davidson, {Cheryl B.} and Katz, {Aubrey J.} and Edelson, {Paul J.}",

year = "1983",

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AU - Winter, Harland S.

AU - Cole, F. Sessions

AU - Huffer, Linda M.

AU - Davidson, Cheryl B.

AU - Katz, Aubrey J.

AU - Edelson, Paul J.

PY - 1983/8

Y1 - 1983/8

N2 - The evaluation of mucosal cellular immune function in the gastrointestinal tract has focused on properties of lymphocytes. We describe methods for use in guinea pig and human tissue that will now permit the maintenance and in vitro study of intestinal macrophages. This study characterizes the resident gastrointestinal macrophage population in the two species and shows that morphologic and ultra-structural characteristics are similar to macrophages from other tissues. Histochemically, the cells are esterase positive and peroxidase negative. They possess surface receptors for immunoglobulin G and complement, and are phagocytic via the Fc receptor. In addition, guinea pig intestinal macrophages synthesize both the second and fourth components of complement.

AB - The evaluation of mucosal cellular immune function in the gastrointestinal tract has focused on properties of lymphocytes. We describe methods for use in guinea pig and human tissue that will now permit the maintenance and in vitro study of intestinal macrophages. This study characterizes the resident gastrointestinal macrophage population in the two species and shows that morphologic and ultra-structural characteristics are similar to macrophages from other tissues. Histochemically, the cells are esterase positive and peroxidase negative. They possess surface receptors for immunoglobulin G and complement, and are phagocytic via the Fc receptor. In addition, guinea pig intestinal macrophages synthesize both the second and fourth components of complement.

UR - http://www.scopus.com/inward/record.url?scp=0020576928&partnerID=8YFLogxK

U2 - 10.1016/0016-5085(83)90324-4

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SP - 358

EP - 363

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 2

ER -

Isolation and characterization of resident macrophages from guinea pig and human intestine

Abstract

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