Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

Swagata Das; Biswajit Mishra; Kamaldeep Gill; Md Saquib Ashraf; Abhay Kumar Singh; Mou Sinha; Sujata Sharma; Immaculata Xess; Krishna Dalal; Tej Pal Singh; Sharmistha Dey

doi:10.1016/j.ijbiomac.2010.09.010

Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

Swagata Das
, Biswajit Mishra
, Kamaldeep Gill
, Md Saquib Ashraf
, Abhay Kumar Singh
, Mou Sinha
, Sujata Sharma
, Immaculata Xess
, Krishna Dalal
, Tej Pal Singh
, Sharmistha Dey

Department of Radiation Oncology

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.

Original language	English
Pages (from-to)	38-43
Number of pages	6
Journal	International Journal of Biological Macromolecules
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ijbiomac.2010.09.010
State	Published - Jan 1 2011

Keywords

Aloe vera
Anti-fungal
Hemagglutination
Inflammation
Surface plasmon resonance

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10.1016/j.ijbiomac.2010.09.010

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Das, S., Mishra, B., Gill, K., Ashraf, M. S., Singh, A. K., Sinha, M., Sharma, S., Xess, I., Dalal, K., Singh, T. P., & Dey, S. (2011). Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel. International Journal of Biological Macromolecules, 48(1), 38-43. https://doi.org/10.1016/j.ijbiomac.2010.09.010

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title = "Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel",

abstract = "The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84\% and 73\% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.",

keywords = "Aloe vera, Anti-fungal, Hemagglutination, Inflammation, Surface plasmon resonance",

author = "Swagata Das and Biswajit Mishra and Kamaldeep Gill and Ashraf, \{Md Saquib\} and Singh, \{Abhay Kumar\} and Mou Sinha and Sujata Sharma and Immaculata Xess and Krishna Dalal and Singh, \{Tej Pal\} and Sharmistha Dey",

year = "2011",

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doi = "10.1016/j.ijbiomac.2010.09.010",

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AU - Das, Swagata

AU - Mishra, Biswajit

AU - Gill, Kamaldeep

AU - Ashraf, Md Saquib

AU - Singh, Abhay Kumar

AU - Sinha, Mou

AU - Sharma, Sujata

AU - Xess, Immaculata

AU - Dalal, Krishna

AU - Singh, Tej Pal

AU - Dey, Sharmistha

PY - 2011/1/1

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N2 - The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.

AB - The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.

KW - Aloe vera

KW - Anti-fungal

KW - Hemagglutination

KW - Inflammation

KW - Surface plasmon resonance

UR - https://www.scopus.com/pages/publications/78650241588

U2 - 10.1016/j.ijbiomac.2010.09.010

DO - 10.1016/j.ijbiomac.2010.09.010

M3 - Article

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AN - SCOPUS:78650241588

SN - 0141-8130

VL - 48

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EP - 43

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 1

ER -

Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

Abstract

Keywords

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