Isoprothrombin, a protein antigenically related to prothrombin, which accumulates in the hepatic endoplasmic reticulum of vitamin K-deficient or warfarin-treated rats, has been isolated by affinity chromatography employing rat prothrombin antibodies linked to Sepharose. Isoprothrombin has the same molecular weight as prothrombin but a different mobility on disc gel electrophoresis, is not barium adsorbable nor activatable to thrombin by factor Xa. Isoprothrombin is converted to thrombin by Echis carinatus venom through the same intermediates as prothrombin.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 1 1973|