Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria

T. Geoffrey Flynn; Anoop Brar; Linda Tremblay; Inder Sarda; Christina Lyons; Donald B. Jennings

doi:10.1016/0006-291X(89)92675-2

Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria

T. Geoffrey Flynn, Anoop Brar, Linda Tremblay, Inder Sarda, Christina Lyons, Donald B. Jennings

Division of Infectious Disease

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Abstract

Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rANP. Amino acid sequence differences show that iso-rANP is genetically distinct from rANP. Iso-rANP has a single disulfide bond between residues 23-39 and this portion of the peptide shows substantial homology to rANP and to porcine brain natriuretic peptide (BNP). Little homology is evident at the N- and C-termini of iso-rANP and ANP. Iso-rANP is equipotent with rANP in eliciting diuresis, natriuresis and hypotension in the bioassay rat.

Original language	English
Pages (from-to)	830-837
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(89)92675-2
State	Published - Jun 15 1989

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title = "Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria",

abstract = "Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rANP. Amino acid sequence differences show that iso-rANP is genetically distinct from rANP. Iso-rANP has a single disulfide bond between residues 23-39 and this portion of the peptide shows substantial homology to rANP and to porcine brain natriuretic peptide (BNP). Little homology is evident at the N- and C-termini of iso-rANP and ANP. Iso-rANP is equipotent with rANP in eliciting diuresis, natriuresis and hypotension in the bioassay rat.",

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T1 - Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria

AU - Flynn, T. Geoffrey

AU - Brar, Anoop

AU - Tremblay, Linda

AU - Sarda, Inder

AU - Lyons, Christina

AU - Jennings, Donald B.

PY - 1989/6/15

Y1 - 1989/6/15

N2 - Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rANP. Amino acid sequence differences show that iso-rANP is genetically distinct from rANP. Iso-rANP has a single disulfide bond between residues 23-39 and this portion of the peptide shows substantial homology to rANP and to porcine brain natriuretic peptide (BNP). Little homology is evident at the N- and C-termini of iso-rANP and ANP. Iso-rANP is equipotent with rANP in eliciting diuresis, natriuresis and hypotension in the bioassay rat.

AB - Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rANP. Amino acid sequence differences show that iso-rANP is genetically distinct from rANP. Iso-rANP has a single disulfide bond between residues 23-39 and this portion of the peptide shows substantial homology to rANP and to porcine brain natriuretic peptide (BNP). Little homology is evident at the N- and C-termini of iso-rANP and ANP. Iso-rANP is equipotent with rANP in eliciting diuresis, natriuresis and hypotension in the bioassay rat.

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U2 - 10.1016/0006-291X(89)92675-2

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JO - Biochemical and Biophysical Research Communications

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IS - 2

ER -

Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria

Abstract

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