Isocratic high-performance liquid chromatography separation of phosphoglycerides and lysophosphoglycerides

Richard W. Gross, Burton E. Sobel

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

An isocratic, rapid method for separation of phospho- and lysophospholipids by high-performance liquid chromatography employing acetonitrile, methanol and water as the mobile phase is described. Separation is achieved on a silica based cation-exchange column with individual components monitored directly by UV absorbance at 203 nm. In solutions of phospho- and lysophospholipid standards and in extracts of rabbit myocardium, recovery of individual components exceeds 95%. With the exception of phosphatidyl serine and phosphatidyl ethanolamine that could not be resolved other major phospho- and lysophospholipid constituents found in extracts of cell membranes are separated completely within 40 min. Retention time for selected moieties can be shortened even further with flow programming.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalJournal of Chromatography A
Volume197
Issue number1
DOIs
StatePublished - 1980

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