Abstract
We introduce the first implementation of a method for the continuous-flow sorting of cells based specifically upon differences in their electrical properties. The method, which we call iso-dielectric separation (IDS), uses the dielectrophoretic (DEP) force in a liquid of spatially varying conductivity to map any electrically distinguishable phenotype to a unique position along the width of a microfluidic channel. The method is analogous to iso-electric focusing, with dielectric properties replacing surface charge as the basis for separation. IDS leverages the correspondence between the physiological state of a cell and its electrical properties to separate cells based upon such characteristics as viability or production of biomolecules [1]. Because IDS selects cells according to their generic electrical differences, it is possible to screen for such production without regard to the specific molecule being produced.
| Original language | English |
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| Pages | 1058-1060 |
| Number of pages | 3 |
| State | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006 |
Conference
| Conference | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 11/5/06 → 11/9/06 |
Keywords
- Cell separations
- Conductivity gradient
- Continuous-flow screening
- Dielectrophoresis