TY - JOUR
T1 - Ischemia-reperfusion induces G-CSF gene expression by renal medullary thick ascending limb cells in vivo and in vitro
AU - Zhang, Ying
AU - Woodward, Vanessa K.
AU - Shelton, John M.
AU - Richardson, James A.
AU - Zhou, Xin J.
AU - Link, Daniel
AU - Kielar, Mariusz L.
AU - Jeyarajah, D. Rohan
AU - Lu, Christopher Y.
PY - 2004/6
Y1 - 2004/6
N2 - Ischemic acute renal failure involves not only the kidney but also extrarenal organs such as the bone marrow that produces inflammatory cells. By ELISA and RNase protection assays, we now show that renal ischemia-reperfusion increases serum concentrations of granulocyte macrophage colony-stimulating factor (G-CSF) protein and increases both G-CSF mRNA and protein in the ischemic kidney. In situ hybridization localized the increased G-CSF mRNA to tubule cells, including medullary thick ascending limb cells (mTAL), in the outer medulla. We also show that mTAL produce G-CSF protein and increase G-CSF mRNA after stimulation by reactive oxygen species in vitro. The production of G-CSF by the kidney after ischemia-reperfusion provides a means of communication from the injured kidney to the bone marrow. This supports the known inflammatory response to ischemia.
AB - Ischemic acute renal failure involves not only the kidney but also extrarenal organs such as the bone marrow that produces inflammatory cells. By ELISA and RNase protection assays, we now show that renal ischemia-reperfusion increases serum concentrations of granulocyte macrophage colony-stimulating factor (G-CSF) protein and increases both G-CSF mRNA and protein in the ischemic kidney. In situ hybridization localized the increased G-CSF mRNA to tubule cells, including medullary thick ascending limb cells (mTAL), in the outer medulla. We also show that mTAL produce G-CSF protein and increase G-CSF mRNA after stimulation by reactive oxygen species in vitro. The production of G-CSF by the kidney after ischemia-reperfusion provides a means of communication from the injured kidney to the bone marrow. This supports the known inflammatory response to ischemia.
KW - Acute renal failure
KW - Granulocyte colony-stimulating factor
KW - Ischemia
KW - Medullary thick ascending limb tubules
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=2442675326&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.00379.2002
DO - 10.1152/ajprenal.00379.2002
M3 - Article
C2 - 14734360
AN - SCOPUS:2442675326
SN - 1931-857X
VL - 286
SP - F1193-F1201
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 6 55-6
ER -