iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies

  • Daniel W. Simmons
  • , Nathaniel Huebsch

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

Micro-heart muscle arrays enable medium-throughput experiments to model the cardiac response to a variety of environmental and pharmaceutical effects. Here, we describe stem cell culture maintenance, methods for successful cardiac differentiation, and formation of micro-heart muscle arrays for electrophysiology and molecular biology assays.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-131
Number of pages21
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2485
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cardiac differentiation
  • Electrophysiology
  • Induced pluripotent stem cells (iPSC)
  • Micro-heart muscle (μHM)
  • Pharmacology

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