Iothalamate stimulates hydroperoxide formation by soybean lipoxygenase

Aubrey R. Morrison; Wendel Brown; Nabil Tauk

doi:10.1016/0090-6980(84)90012-1

Iothalamate stimulates hydroperoxide formation by soybean lipoxygenase

Aubrey R. Morrison, Wendel Brown, Nabil Tauk

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Abstract

Sodium iothalamate produced a dose dependent increase in basal oxygen consumption when soybean lipoxygenase was incubated with arachidonic acid in 0.1M borate buffer pH 9.0. The increase in oxygen consumption was associated with an increase in absorbance at 234 nm indicating an increased conjugated diene formation. The stimulation of O₂ consumption was demonstrated to be due to an increase in 1500H arachidonic formation. The increase in 1500H arachidonate formation could be blocked by mannitol which is an inhibitor of the lipoxygenase enzyme. N-methyl glucamine (meglumine) which is added to some preparations of iothalamate, was also able to suppress the increase in hydroperoxide formation in a dose dependent fashion.

Original language	English
Pages (from-to)	753-759
Number of pages	7
Journal	Prostaglandins
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/0090-6980(84)90012-1
State	Published - May 1984

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title = "Iothalamate stimulates hydroperoxide formation by soybean lipoxygenase",

abstract = "Sodium iothalamate produced a dose dependent increase in basal oxygen consumption when soybean lipoxygenase was incubated with arachidonic acid in 0.1M borate buffer pH 9.0. The increase in oxygen consumption was associated with an increase in absorbance at 234 nm indicating an increased conjugated diene formation. The stimulation of O2 consumption was demonstrated to be due to an increase in 1500H arachidonic formation. The increase in 1500H arachidonate formation could be blocked by mannitol which is an inhibitor of the lipoxygenase enzyme. N-methyl glucamine (meglumine) which is added to some preparations of iothalamate, was also able to suppress the increase in hydroperoxide formation in a dose dependent fashion.",

author = "Morrison, {Aubrey R.} and Wendel Brown and Nabil Tauk",

note = "Funding Information: The work is supported by NIH research grants PO1 Air109976and AM30542-32. Dr. Xorrison is an established investigator of the American Heart Association.",

year = "1984",

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doi = "10.1016/0090-6980(84)90012-1",

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T1 - Iothalamate stimulates hydroperoxide formation by soybean lipoxygenase

AU - Morrison, Aubrey R.

AU - Brown, Wendel

AU - Tauk, Nabil

N1 - Funding Information: The work is supported by NIH research grants PO1 Air109976and AM30542-32. Dr. Xorrison is an established investigator of the American Heart Association.

PY - 1984/5

Y1 - 1984/5

N2 - Sodium iothalamate produced a dose dependent increase in basal oxygen consumption when soybean lipoxygenase was incubated with arachidonic acid in 0.1M borate buffer pH 9.0. The increase in oxygen consumption was associated with an increase in absorbance at 234 nm indicating an increased conjugated diene formation. The stimulation of O2 consumption was demonstrated to be due to an increase in 1500H arachidonic formation. The increase in 1500H arachidonate formation could be blocked by mannitol which is an inhibitor of the lipoxygenase enzyme. N-methyl glucamine (meglumine) which is added to some preparations of iothalamate, was also able to suppress the increase in hydroperoxide formation in a dose dependent fashion.

AB - Sodium iothalamate produced a dose dependent increase in basal oxygen consumption when soybean lipoxygenase was incubated with arachidonic acid in 0.1M borate buffer pH 9.0. The increase in oxygen consumption was associated with an increase in absorbance at 234 nm indicating an increased conjugated diene formation. The stimulation of O2 consumption was demonstrated to be due to an increase in 1500H arachidonic formation. The increase in 1500H arachidonate formation could be blocked by mannitol which is an inhibitor of the lipoxygenase enzyme. N-methyl glucamine (meglumine) which is added to some preparations of iothalamate, was also able to suppress the increase in hydroperoxide formation in a dose dependent fashion.

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U2 - 10.1016/0090-6980(84)90012-1

DO - 10.1016/0090-6980(84)90012-1

M3 - Article

C2 - 6431500

AN - SCOPUS:0021236260

SN - 0090-6980

VL - 27

SP - 753

EP - 759

JO - Prostaglandins

JF - Prostaglandins

IS - 5

ER -

Iothalamate stimulates hydroperoxide formation by soybean lipoxygenase

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