Ionizing radiation activates transcription of the EGR1 gene via CArG elements

R. Datta; E. Rubin; V. Sukhatme; S. Qureshi; D. Hallahan; R. R. Weichselbaum; D. W. Kufe

Ionizing radiation activates transcription of the EGR1 gene via CArG elements

R. Datta, E. Rubin, V. Sukhatme, S. Qureshi, D. Hallahan, R. R. Weichselbaum, D. W. Kufe

Research output: Contribution to journal › Article › peer-review

Abstract

The present studies have examined the effects of ionizing radiation on control of the early growth response 1 (EGR1) gene. Exposure of human HL-525 cells to x-rays was associated with increases in EGR1 mRNA levels. Nuclear run-on assays showed that this effect is related at least in part to activation of EGR1 gene transcription. Sequences responsive to ionizing radiation-induced signals were determined by deletion analysis of the EGR1 promoter. The results demonstrate that x-ray inducibility of the EGR1 gene is conferred by a region containing six serum response or CC(A+T-rich)₆GG (CArG) motifs. Further analysis confirmed that the region encompassing the three distal or upstream CArG elements is functional in the x-ray response. Moreover, this region conferred x-ray inducibility to a minimal thymidine kinase gene promoter. Taken together, these results indicate that ionizing radiation induces EGR1 transcription through CArG elements.

Original language	English
Pages (from-to)	10149-10153
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	21
State	Published - 1992

Keywords

Early growth response
Serum response element
X-rays

Cite this

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title = "Ionizing radiation activates transcription of the EGR1 gene via CArG elements",

abstract = "The present studies have examined the effects of ionizing radiation on control of the early growth response 1 (EGR1) gene. Exposure of human HL-525 cells to x-rays was associated with increases in EGR1 mRNA levels. Nuclear run-on assays showed that this effect is related at least in part to activation of EGR1 gene transcription. Sequences responsive to ionizing radiation-induced signals were determined by deletion analysis of the EGR1 promoter. The results demonstrate that x-ray inducibility of the EGR1 gene is conferred by a region containing six serum response or CC(A+T-rich)6GG (CArG) motifs. Further analysis confirmed that the region encompassing the three distal or upstream CArG elements is functional in the x-ray response. Moreover, this region conferred x-ray inducibility to a minimal thymidine kinase gene promoter. Taken together, these results indicate that ionizing radiation induces EGR1 transcription through CArG elements.",

keywords = "Early growth response, Serum response element, X-rays",

author = "R. Datta and E. Rubin and V. Sukhatme and S. Qureshi and D. Hallahan and Weichselbaum, {R. R.} and Kufe, {D. W.}",

year = "1992",

language = "English",

volume = "89",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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TY - JOUR

T1 - Ionizing radiation activates transcription of the EGR1 gene via CArG elements

AU - Datta, R.

AU - Rubin, E.

AU - Sukhatme, V.

AU - Qureshi, S.

AU - Hallahan, D.

AU - Weichselbaum, R. R.

AU - Kufe, D. W.

PY - 1992

Y1 - 1992

N2 - The present studies have examined the effects of ionizing radiation on control of the early growth response 1 (EGR1) gene. Exposure of human HL-525 cells to x-rays was associated with increases in EGR1 mRNA levels. Nuclear run-on assays showed that this effect is related at least in part to activation of EGR1 gene transcription. Sequences responsive to ionizing radiation-induced signals were determined by deletion analysis of the EGR1 promoter. The results demonstrate that x-ray inducibility of the EGR1 gene is conferred by a region containing six serum response or CC(A+T-rich)6GG (CArG) motifs. Further analysis confirmed that the region encompassing the three distal or upstream CArG elements is functional in the x-ray response. Moreover, this region conferred x-ray inducibility to a minimal thymidine kinase gene promoter. Taken together, these results indicate that ionizing radiation induces EGR1 transcription through CArG elements.

AB - The present studies have examined the effects of ionizing radiation on control of the early growth response 1 (EGR1) gene. Exposure of human HL-525 cells to x-rays was associated with increases in EGR1 mRNA levels. Nuclear run-on assays showed that this effect is related at least in part to activation of EGR1 gene transcription. Sequences responsive to ionizing radiation-induced signals were determined by deletion analysis of the EGR1 promoter. The results demonstrate that x-ray inducibility of the EGR1 gene is conferred by a region containing six serum response or CC(A+T-rich)6GG (CArG) motifs. Further analysis confirmed that the region encompassing the three distal or upstream CArG elements is functional in the x-ray response. Moreover, this region conferred x-ray inducibility to a minimal thymidine kinase gene promoter. Taken together, these results indicate that ionizing radiation induces EGR1 transcription through CArG elements.

KW - Early growth response

KW - Serum response element

KW - X-rays

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M3 - Article

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AN - SCOPUS:0026452858

SN - 0027-8424

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Ionizing radiation activates transcription of the EGR1 gene via CArG elements

Abstract

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