Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism

James J. Walters; Michael A. Grayson; Michael L. Gross; Maureen Hughes; Georgia Shearer; Daniel H. Kohl; James Bashkin

doi:10.1016/j.jasms.2005.10.004

Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism

James J. Walters
, Michael A. Grayson
, Michael L. Gross
, Maureen Hughes
, Georgia Shearer
, Daniel H. Kohl
, James Bashkin

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The aim of this work is to establish a quantitative method to determine the ratio of [U-¹³C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.

Original language	English
Pages (from-to)	104-107
Number of pages	4
Journal	Journal of the American Society for Mass Spectrometry
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/j.jasms.2005.10.004
State	Published - Jan 2006

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title = "Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism",

abstract = "The aim of this work is to establish a quantitative method to determine the ratio of [U-13C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.",

author = "Walters, \{James J.\} and Grayson, \{Michael A.\} and Gross, \{Michael L.\} and Maureen Hughes and Georgia Shearer and Kohl, \{Daniel H.\} and James Bashkin",

note = "Funding Information: This work was supported by the NCRR of the NIH (grant P01RR00954) and the NSF (grant NSF MCB-0200490).",

year = "2006",

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doi = "10.1016/j.jasms.2005.10.004",

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T1 - Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism

AU - Walters, James J.

AU - Grayson, Michael A.

AU - Gross, Michael L.

AU - Hughes, Maureen

AU - Shearer, Georgia

AU - Kohl, Daniel H.

AU - Bashkin, James

N1 - Funding Information: This work was supported by the NCRR of the NIH (grant P01RR00954) and the NSF (grant NSF MCB-0200490).

PY - 2006/1

Y1 - 2006/1

N2 - The aim of this work is to establish a quantitative method to determine the ratio of [U-13C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.

AB - The aim of this work is to establish a quantitative method to determine the ratio of [U-13C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.

UR - https://www.scopus.com/pages/publications/29744445319

U2 - 10.1016/j.jasms.2005.10.004

DO - 10.1016/j.jasms.2005.10.004

M3 - Article

C2 - 16352445

AN - SCOPUS:29744445319

SN - 1044-0305

VL - 17

SP - 104

EP - 107

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

IS - 1

ER -

Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism

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