TY - JOUR
T1 - Iodination of arachidonic acid mediated by eosinophil peroxidase, myeloperoxidase and lactoperoxidase identification and comparison of products
AU - Turk, John
AU - Henderson, William R.
AU - Klebanoff, Seymour J.
AU - Hubbard, Walter C.
N1 - Funding Information:
This work was supported by grants GM 15431 , AM 28511, AI 17758 and AI 07763 from the National Institutes of Health and by a grant from the Rockefeller Foundation. J.T. was supported by grant GM 07569 and W.R. H. is the recipient of an Allergic Diseases Academic Award AI 00487 from the National Institute of Allergy and Infectious Diseases. The invaluable technical assistance of Gertrude Chiang, John Lawson, Mark Phillips, Ella Stitt and Ann Waltersdorph and the secretarial assistance of Janice Neely are gratefully acknowledged. The advice and interest of Dr. John Oates is much appreciated.
PY - 1983/4/13
Y1 - 1983/4/13
N2 - Arachidonic acid undergoes iodination in the presence of hydrogen peroxide, iodide, and either eosinophil peroxidase, myeloperoxidase or lactoperoxidase. The profile of products generated by each of the three peroxidases is similar as determined by reversed-phase high-performance liquid chromatography. Structural analysis of the products indicate that: 1, each of the four double bonds in arachidonic acid is susceptible to iodination; 2, arachidonic acid can be multiply iodinated; and 3, the carboxylate moiety does not participate in the formation of all products. The isomeric composition of the isolated products indicates that peroxidase-mediated iodination of arachidonate is not stereoselective.
AB - Arachidonic acid undergoes iodination in the presence of hydrogen peroxide, iodide, and either eosinophil peroxidase, myeloperoxidase or lactoperoxidase. The profile of products generated by each of the three peroxidases is similar as determined by reversed-phase high-performance liquid chromatography. Structural analysis of the products indicate that: 1, each of the four double bonds in arachidonic acid is susceptible to iodination; 2, arachidonic acid can be multiply iodinated; and 3, the carboxylate moiety does not participate in the formation of all products. The isomeric composition of the isolated products indicates that peroxidase-mediated iodination of arachidonate is not stereoselective.
KW - Arachidonic acid
KW - Iodination
KW - Lactoperoxidase
KW - Myeloperoxidase
KW - Peroxidase
UR - http://www.scopus.com/inward/record.url?scp=0021102118&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(83)90173-X
DO - 10.1016/0005-2760(83)90173-X
M3 - Article
C2 - 6299368
AN - SCOPUS:0021102118
VL - 751
SP - 189
EP - 200
JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
SN - 0005-2760
IS - 2
ER -