TY - JOUR
T1 - Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types
AU - Nakano, Takanari
AU - Shimanuki, Tomoko
AU - Matsushita, Makoto
AU - Koyama, Iwao
AU - Inoue, Ikuo
AU - Katayama, Shigehiro
AU - Alpers, David H.
AU - Komoda, Tsugikazu
N1 - Funding Information:
The authors are grateful to Dr. Masaaki Kojima (Sibayagi Co., Gunma) for his technical assistance and Misses Akiko Kawano and Rina Shinozaki for her secretarial assistance. This work was supported by a grant from Otsuka Pharmaceutical Co. Ltd. (Tokyo).
PY - 2006/3/3
Y1 - 2006/3/3
N2 - Serum levels of intestinal alkaline phosphatase (IAP), a protein implicated in transcellular transport of chylomicrons, vary among ABO blood groups. In rat enterocytes, IAP is associated with chylomicron secretion, but the rat expresses only blood group A. It is not known whether chylomicron secretion may be affected in humans who express multiple blood group types. Serum samples from 40 healthy subjects were obtained after overnight fast and 3 h after a high-fat meal, and assayed for IAP and apolipoprotein B-48 (apoB-48), both proteins exclusive to intestine, although only apoB-48 is found in chylomicrons. The two proteins were greater in subjects without blood antigen A (B and O) than in those with this antigen (A and AB); 2.4- and 4.7-fold for IAP and 1.5- and 2.0-fold for apoB-48 before and after the meal, respectively. Moreover, IAP and apoB-48 levels were strongly correlated in the subjects with the secretor phenotype (r > 0.81). These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. In addition, ABO blood type classification in apoB-48 measurement would improve the diagnostic value in the evaluation of metabolic syndrome.
AB - Serum levels of intestinal alkaline phosphatase (IAP), a protein implicated in transcellular transport of chylomicrons, vary among ABO blood groups. In rat enterocytes, IAP is associated with chylomicron secretion, but the rat expresses only blood group A. It is not known whether chylomicron secretion may be affected in humans who express multiple blood group types. Serum samples from 40 healthy subjects were obtained after overnight fast and 3 h after a high-fat meal, and assayed for IAP and apolipoprotein B-48 (apoB-48), both proteins exclusive to intestine, although only apoB-48 is found in chylomicrons. The two proteins were greater in subjects without blood antigen A (B and O) than in those with this antigen (A and AB); 2.4- and 4.7-fold for IAP and 1.5- and 2.0-fold for apoB-48 before and after the meal, respectively. Moreover, IAP and apoB-48 levels were strongly correlated in the subjects with the secretor phenotype (r > 0.81). These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. In addition, ABO blood type classification in apoB-48 measurement would improve the diagnostic value in the evaluation of metabolic syndrome.
KW - ABO blood group
KW - Apolipoprotein B-48
KW - Chylomicron
KW - Intestinal alkaline phosphatase
KW - Secretor phenotype
KW - Triglyceride
UR - http://www.scopus.com/inward/record.url?scp=30944440425&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.12.145
DO - 10.1016/j.bbrc.2005.12.145
M3 - Article
C2 - 16412386
AN - SCOPUS:30944440425
SN - 0006-291X
VL - 341
SP - 33
EP - 38
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -