TY - JOUR
T1 - Investigating tumor perfusion and metabolism using multiple hyperpolarized 13C compounds
T2 - HP001, pyruvate and urea
AU - Von Morze, Cornelius
AU - Larson, Peder E.Z.
AU - Hu, Simon
AU - Yoshihara, Hikari A.I.
AU - Bok, Robert A.
AU - Goga, Andrei
AU - Ardenkjaer-Larsen, Jan Henrik
AU - Vigneron, Daniel B.
N1 - Funding Information:
We gratefully acknowledge grant support from National Institutes of Health grants P41EB013598 and R01EB007588 . We also acknowledge the assistance of Kristen Scott with the animal studies, as well as the help from Mark Van Criekinge, Galen Reed, Peter Shin and Ilwoo Park. We also thank Kayvan Keshari and David Wilson for helping us with the copolarization procedure.
PY - 2012/4
Y1 - 2012/4
N2 - The metabolically inactive hyperpolarized agents HP001 (bis-1,1-(hydroxymethyl)-[1- 13C]cyclopropane-d 8) and urea enable a new type of perfusion magnetic resonance imaging based on a direct signal source that is background-free. The addition of perfusion information to metabolic information obtained by spectroscopic imaging of hyperpolarized [1- 13C]pyruvate would be of great value in exploring the relationship between perfusion and metabolism in cancer. In preclinical normal murine and cancer model studies, we performed both dynamic multislice imaging of the specialized hyperpolarized perfusion compound HP001 (T 1=95 s ex vivo, 32 s in vivo at 3 T) using a pulse sequence with balanced steady-state free precession and ramped flip angle over time for efficient utilization of the hyperpolarized magnetization and three-dimensional echo-planar spectroscopic imaging of urea copolarized with [1- 13C]pyruvate, with compressed sensing for resolution enhancement. For the dynamic data, peak signal maps and blood flow maps derived from perfusion modeling were generated. The spatial heterogeneity of perfusion was increased 2.9-fold in tumor tissues (P=.05), and slower washout was observed in the dynamic data. The results of separate dynamic HP001 imaging and copolarized pyruvate/urea imaging were compared. A strong and significant correlation (R=0.73, P=.02) detected between the urea and HP001 data confirmed the value of copolarizing urea with pyruvate for simultaneous assessment of perfusion and metabolism.
AB - The metabolically inactive hyperpolarized agents HP001 (bis-1,1-(hydroxymethyl)-[1- 13C]cyclopropane-d 8) and urea enable a new type of perfusion magnetic resonance imaging based on a direct signal source that is background-free. The addition of perfusion information to metabolic information obtained by spectroscopic imaging of hyperpolarized [1- 13C]pyruvate would be of great value in exploring the relationship between perfusion and metabolism in cancer. In preclinical normal murine and cancer model studies, we performed both dynamic multislice imaging of the specialized hyperpolarized perfusion compound HP001 (T 1=95 s ex vivo, 32 s in vivo at 3 T) using a pulse sequence with balanced steady-state free precession and ramped flip angle over time for efficient utilization of the hyperpolarized magnetization and three-dimensional echo-planar spectroscopic imaging of urea copolarized with [1- 13C]pyruvate, with compressed sensing for resolution enhancement. For the dynamic data, peak signal maps and blood flow maps derived from perfusion modeling were generated. The spatial heterogeneity of perfusion was increased 2.9-fold in tumor tissues (P=.05), and slower washout was observed in the dynamic data. The results of separate dynamic HP001 imaging and copolarized pyruvate/urea imaging were compared. A strong and significant correlation (R=0.73, P=.02) detected between the urea and HP001 data confirmed the value of copolarizing urea with pyruvate for simultaneous assessment of perfusion and metabolism.
KW - C13
KW - HP001
KW - Hyperpolarized
KW - Perfusion
KW - Pyruvate
KW - Urea
UR - http://www.scopus.com/inward/record.url?scp=84857114595&partnerID=8YFLogxK
U2 - 10.1016/j.mri.2011.09.026
DO - 10.1016/j.mri.2011.09.026
M3 - Article
C2 - 22169407
AN - SCOPUS:84857114595
SN - 0730-725X
VL - 30
SP - 305
EP - 311
JO - Magnetic Resonance Imaging
JF - Magnetic Resonance Imaging
IS - 3
ER -