Lymphocyte rolling velocity is determined largely by interactions between leukocyte α4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.

Original languageEnglish
Pages (from-to)H2213-H2226
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number6 53-6
StatePublished - Jun 1 2003


  • Cell adhesion molecule
  • Hemodynamic parameters
  • Lymphocyte rolling
  • Microvascular vessels


Dive into the research topics of 'Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node'. Together they form a unique fingerprint.

Cite this