Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy

Song Hu, Ping Yan, Konstantin Maslov, Jin Moo Lee, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We report optical-resolution photoacoustic microscopy (OR-PAM) for in vivo imaging of amyloid plaques in an Alzheimer's disease mouse model. Validation using conventional fluorescence microscopy and multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window without angiographic contrast agents. OR-PAM, capable of providing both exogenous molecular contrast and endogenous hemoglobin contrast, has the potential to serve as a new technology for in vivo microscopic observations of cerebral plaque deposits.

Original languageEnglish
Pages (from-to)3899-3901
Number of pages3
JournalOptics Letters
Volume34
Issue number24
DOIs
StatePublished - Dec 15 2009

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