Intratumoral Cell Neighborhoods Coordinate Outcomes in Pancreatic Ductal Adenocarcinoma

Max M. Wattenberg, Sarah Colby, Ignacio Garrido-Laguna, Yuqing Xue, Renee Chang, Devora Delman, Jesse Lee, Kajsa Affolter, Sean J. Mulvihill, M. Shaalan Beg, Andrea Wang-Gillam, James Lloyd Wade, Katherine A. Guthrie, E. Gabriela Chiorean, Syed A. Ahmad, Andrew M. Lowy, Philip Agop Philip, Davendra P.S. Sohal, Gregory L. Beatty

Research output: Contribution to journalArticlepeer-review

Abstract

Background & Aims: Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease characterized by a spatially heterogeneous tumor microenvironment. Within the PDA microenvironment, cells organize into communities where cell fate is influenced by neighboring cells of diverse ontogeny and function. However, it remains unclear how cell neighborhoods in the tumor microenvironment evolve with treatment and impact clinical outcomes. Methods: Here, using automated chromogenic multiplex immunohistochemistry and unsupervised computational image analysis of human PDA tumors, we investigated cell neighborhoods in surgically resected tumors from patients with chemotherapy-naïve PDA (n = 59) and neoadjuvant chemotherapy-treated PDA (n = 57). Single cells were defined by lineage markers (CD3, CD8, Foxp3, CD68, CK19), proliferation (Ki67), and neighboring cells. Results: Distinct intratumoral immune and tumor cell subsets were defined by neighboring cells. Higher content of stromal-associated macrophages was seen in chemotherapy-naïve tumors from long-term survivors (overall survival >3 years) compared with short-term survivors (overall survival <1 year), whereas immune-excluded tumor cells were higher in short-term survivors. Chemotherapy-treated vs -naïve tumors showed lower content of tumor-associated T cells and macrophages but similar densities of stromal-associated immune cells. However, proliferating tumor cell subsets with immune-rich neighborhoods were higher in chemotherapy-treated tumors. In a blinded analysis of tumors from patients treated with neoadjuvant chemotherapy, a composite index comprising lower quantities of immune-excluded tumor cells and higher spatially distinct immune cell subsets was associated with prolonged survival. Conclusions: Together, these data provide new insights into discrete cell communities in PDA and show their clinical relevance.

Original languageEnglish
Pages (from-to)1114-1129
Number of pages16
JournalGastroenterology
Volume166
Issue number6
DOIs
StatePublished - Jun 2024

Keywords

  • Biomarkers
  • Immune Cells
  • Inflammation
  • Pancreatic Cancer
  • Tumor Microenvironment

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