TY - JOUR
T1 - Intracellular receptor sorting during endocytosis
T2 - Comparative immunoelectron microscopy of multiple receptors in rat liver
AU - Geuze, Hans J.
AU - Slot, Jan W.
AU - Strous, Ger J.A.M.
AU - Peppard, Jane
AU - von Figura, Kurt
AU - Hasilik, Andrej
AU - Schwartz, Alan L.
N1 - Funding Information:
The study was supported in part by grants from the Koningin Wilhelmina Fonds. The Netherlands. the National Foundation (Basil O’Connor Research Award), the National Institutes of Health. USA (GM 32477) the Deutsche Forschungsgemeinschaft and Fonds der Chemischen Industrie. A. L. S. was supported in part by a John A. and George Hartford Foundation Fellowship.
PY - 1984/5
Y1 - 1984/5
N2 - Using double-label quantitative immunoelectron microscopy on ultrathin cryosections of rat liver, we have compared the endocytotic pathways of the receptors for asialoglycoprotein (ASGP-R), mannose-6-phosphate ligands (MP-R), and polymeric IgA (IgA-R). All three were found within the Golgi complex, along the entire plasma membrane, in coated pits and vesicles, and within a compartment of uncoupling of receptors and ligand (CURL). The receptors occurred randomly at the cell surface, in coated pits and vesicles. Within CURL tubules ASGP-R and MP-R were colocalized, but IgA-R and ASGP-R displayed dramatic microheterogeneity. Thus, in addition to its role in uncoupling and sorting recycling receptor from ligand, CURL serves as a compartment to segregate recycling receptor (e.g. ASGP-R) from receptor involved in transcytosis (e.g. IgA-R).
AB - Using double-label quantitative immunoelectron microscopy on ultrathin cryosections of rat liver, we have compared the endocytotic pathways of the receptors for asialoglycoprotein (ASGP-R), mannose-6-phosphate ligands (MP-R), and polymeric IgA (IgA-R). All three were found within the Golgi complex, along the entire plasma membrane, in coated pits and vesicles, and within a compartment of uncoupling of receptors and ligand (CURL). The receptors occurred randomly at the cell surface, in coated pits and vesicles. Within CURL tubules ASGP-R and MP-R were colocalized, but IgA-R and ASGP-R displayed dramatic microheterogeneity. Thus, in addition to its role in uncoupling and sorting recycling receptor from ligand, CURL serves as a compartment to segregate recycling receptor (e.g. ASGP-R) from receptor involved in transcytosis (e.g. IgA-R).
UR - https://www.scopus.com/pages/publications/0021233676
U2 - 10.1016/0092-8674(84)90315-5
DO - 10.1016/0092-8674(84)90315-5
M3 - Article
C2 - 6327050
AN - SCOPUS:0021233676
SN - 0092-8674
VL - 37
SP - 195
EP - 204
JO - Cell
JF - Cell
IS - 1
ER -