Intracellular receptor sorting during endocytosis: Comparative immunoelectron microscopy of multiple receptors in rat liver

Hans J. Geuze, Jan W. Slot, Ger J.A.M. Strous, Jane Peppard, Kurt von Figura, Andrej Hasilik, Alan L. Schwartz

Research output: Contribution to journalArticlepeer-review

204 Scopus citations

Abstract

Using double-label quantitative immunoelectron microscopy on ultrathin cryosections of rat liver, we have compared the endocytotic pathways of the receptors for asialoglycoprotein (ASGP-R), mannose-6-phosphate ligands (MP-R), and polymeric IgA (IgA-R). All three were found within the Golgi complex, along the entire plasma membrane, in coated pits and vesicles, and within a compartment of uncoupling of receptors and ligand (CURL). The receptors occurred randomly at the cell surface, in coated pits and vesicles. Within CURL tubules ASGP-R and MP-R were colocalized, but IgA-R and ASGP-R displayed dramatic microheterogeneity. Thus, in addition to its role in uncoupling and sorting recycling receptor from ligand, CURL serves as a compartment to segregate recycling receptor (e.g. ASGP-R) from receptor involved in transcytosis (e.g. IgA-R).

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalCell
Volume37
Issue number1
DOIs
StatePublished - May 1984

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