TY - JOUR
T1 - Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts.
AU - Teti, A.
AU - Grano, M.
AU - Teitelbaum, S. L.
AU - Hruska, K. A.
AU - Colucci, S.
AU - Zambonin Zallone, A.
PY - 1989/8/1
Y1 - 1989/8/1
N2 - In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]. The decrease of [Ca2+], during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pH1 can modulate osteoclast activity together with modifications of [Ca2+], probably acting as intracellular signals.
AB - In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]. The decrease of [Ca2+], during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pH1 can modulate osteoclast activity together with modifications of [Ca2+], probably acting as intracellular signals.
UR - http://www.scopus.com/inward/record.url?scp=0024712333&partnerID=8YFLogxK
M3 - Article
C2 - 2803732
AN - SCOPUS:0024712333
SN - 0037-8771
VL - 65
SP - 689
EP - 693
JO - Bollettino della Societa italiana di biologia sperimentale
JF - Bollettino della Societa italiana di biologia sperimentale
IS - 8
ER -