TY - JOUR
T1 - Interplay of loss of ERK dependence and amplification of apoptotic signals in arsenic treated rat hepatocytes
AU - Ray, Atish
AU - Chatterjee, Sarmishtha
AU - Mukherjee, Sandip
AU - Bhattacharya, Shelley
PY - 2013/12
Y1 - 2013/12
N2 - The present study was designed to demonstrate the loss of ERK dependence on amplified apoptotic signals in arsenic treated rat hepatocytes. Arsenic trioxide treatment (10 μM) exhibited simultaneous activation of caspase 9, caspase 3 and caspase 8. Moreover, expression and secretion of Fas ligand was also prominent. Reduction in nuclear translocation of pERK was directly associated with caspase activation. Treatment with ERK inhibitor further augmented caspase activation. An experiment with Fas receptor blocked cells confirmed self amplification of apoptotic signals via Fas R/caspase 8 interaction.
AB - The present study was designed to demonstrate the loss of ERK dependence on amplified apoptotic signals in arsenic treated rat hepatocytes. Arsenic trioxide treatment (10 μM) exhibited simultaneous activation of caspase 9, caspase 3 and caspase 8. Moreover, expression and secretion of Fas ligand was also prominent. Reduction in nuclear translocation of pERK was directly associated with caspase activation. Treatment with ERK inhibitor further augmented caspase activation. An experiment with Fas receptor blocked cells confirmed self amplification of apoptotic signals via Fas R/caspase 8 interaction.
KW - Apoptosis
KW - Arsenic
KW - Caspase
KW - ERK
KW - Fas
KW - Hepatocyte
UR - http://www.scopus.com/inward/record.url?scp=84891456807&partnerID=8YFLogxK
U2 - 10.1007/s40009-013-0175-6
DO - 10.1007/s40009-013-0175-6
M3 - Article
AN - SCOPUS:84891456807
SN - 0250-541X
VL - 36
SP - 599
EP - 602
JO - National Academy Science Letters
JF - National Academy Science Letters
IS - 6
ER -