Inter‐mouse strain differences in the in vivo anti‐CD3 induced cytokine release

C. FERRAN, M. DY, K. SHEEHAN, S. MERITE, R. SCHREIBER, P. LANDAIS, G. GRAU, J. BLUESTONE, J. ‐F BACH, L. CHATENOUD

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42 Scopus citations

Abstract

Triggering of the CD3 molecule by in vivo injection of the hamster anti‐murine CD3 monoclonal antibody 145‐2C11 in adult BALB/c mice leads to massive although transient T cell activation. High levels of tumour necrosis factor (TNF), interferon‐gamma (IFN‐γ), IL‐2, IL‐3 and IL‐6 are released into the circulation 1 to 8 h after a single 10 μg 145‐2C11 i.v. injection. This release induces an impressive self‐limited physical reaction associating hypothermia, hypomotility (as assessed by actimetry), diarrhoea, piloerection and even death when high doses (a single dose of > 100 μg/mouse injection) are administered. In vivo injection of 145‐2C11 to other selected mouse strains, namely NZW, CBA/J and C3H/HeJ, induced both different cytokine release patterns and sickness. 145‐2C11 induced significant release of TNF and IL‐2 in all four strains. At variance. IFN‐γ was only detected in BALB/c mice sera which, in terms of physical reaction (hypothermia and hypomotility) were the most affected. Higher and long‐lasting circulating 1L‐3/GM‐CSF levels were present in CBA/J sera, correlating with a later recovery. These results underline heterogeneity in the in vivo cell activation pattern among different mouse strains, when triggering T lymphocytes via the CD3/Ti molecule as compared to exclusive targeting of monocyte/macrophages by means of lipopolysaccharide.

Original languageEnglish
Pages (from-to)537-543
Number of pages7
JournalClinical & Experimental Immunology
Volume86
Issue number3
DOIs
StatePublished - Dec 1991

Keywords

  • CD3
  • T cell receptor
  • in vivo cell activation
  • monoclonal antibody
  • serotherapy

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