Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells

Dominic Fee, Dana Grzybicki, Matthew Dobbs, Sudakhar Ihyer, Justine Clotfelter, Sinarack MacVilay, Michael N. Hart, Matyas Sandor, Zsuzsanna Fabry

Research output: Contribution to journalArticle

78 Scopus citations


Interleukin 6 (IL-6) is a cytokine that acts on a wide range of tissues influencing cell growth and differentiation. Here we show that IL-6 plays a role in the early vascular development (vasculogenesis) in the central nervous system (CNS). We report that IL-6 induces the proliferation of brain microvascular endothelial cells in vitro. Furthermore, IL-6 significantly accelerates the formation of tube-like structures by these cells in Matrigel basement matrix. Moreover, IL-6 mRNA is expressed in vivo in two physiological conditions in which vascularization in the CNS is important: (1) during normal brain development, (2) during the healing process of a traumatic brain injury. Expression of IL-6 mRNA coincides with the expression of vascular endothelial growth factor (VEGF) mRNA in the developing brain with decreasing expression following birth. However, IL-6 mRNA can be detected in the healing adult murine brain tissue by in situ hybridization coinciding with the period of intense tissue reorganization. The transient upregulation of IL-6 mRNA during normal brain development and at brain injury site and the effect of IL-6 on in vitro vasculogenesis suggest that IL-6 may play a role in normal physiology of vascularization in the CNS. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)655-665
Number of pages11
Issue number6
StatePublished - Jun 2000
Externally publishedYes


  • Brain injury
  • Cytokine
  • Development
  • Growth factor
  • Vascularization

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    Fee, D., Grzybicki, D., Dobbs, M., Ihyer, S., Clotfelter, J., MacVilay, S., Hart, M. N., Sandor, M., & Fabry, Z. (2000). Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells. Cytokine, 12(6), 655-665.