Interleukin-2 binding proteins in sera from normal subjects and multiple sclerosis patients

John L. Trotter; C. A. Damico; A. L. Trotter; K. G. Collins; A. H. Cross

doi:10.1212/WNL.45.11.1971

Interleukin-2 binding proteins in sera from normal subjects and multiple sclerosis patients

John L. Trotter, C. A. Damico, A. L. Trotter, K. G. Collins, A. H. Cross

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Abstract

We previously reported elevations of interleukin 2 (IL-2) in the serum of patients with chronic progressive MS. Using gel chromatography, protein A sepharose afinity chromatography, and ELISAs for IL-2 and the IL-2 soluble receptor, we now demonstrate that this cytokine is bound to serum proteins. These serum proteins include antibodies to IL-2, soluble IL-2 receptors, and high-molecular-weight proteins. Using a CTLL cell assay, a serum fraction corresponding to IgG antibodies to IL-2 inhibited the activity of this cytokine. Thus, we present evidence far potential immunomodulation of a pivotal cytokine in MS by serum proteins.

Original language	English
Pages (from-to)	1971-1974
Number of pages	4
Journal	Neurology
Volume	45
Issue number	11
DOIs	https://doi.org/10.1212/WNL.45.11.1971
State	Published - Nov 1995

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title = "Interleukin-2 binding proteins in sera from normal subjects and multiple sclerosis patients",

abstract = "We previously reported elevations of interleukin 2 (IL-2) in the serum of patients with chronic progressive MS. Using gel chromatography, protein A sepharose afinity chromatography, and ELISAs for IL-2 and the IL-2 soluble receptor, we now demonstrate that this cytokine is bound to serum proteins. These serum proteins include antibodies to IL-2, soluble IL-2 receptors, and high-molecular-weight proteins. Using a CTLL cell assay, a serum fraction corresponding to IgG antibodies to IL-2 inhibited the activity of this cytokine. Thus, we present evidence far potential immunomodulation of a pivotal cytokine in MS by serum proteins.",

author = "Trotter, {John L.} and Damico, {C. A.} and Trotter, {A. L.} and Collins, {K. G.} and Cross, {A. H.}",

year = "1995",

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doi = "10.1212/WNL.45.11.1971",

language = "English",

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AU - Trotter, John L.

AU - Damico, C. A.

AU - Trotter, A. L.

AU - Collins, K. G.

AU - Cross, A. H.

PY - 1995/11

Y1 - 1995/11

N2 - We previously reported elevations of interleukin 2 (IL-2) in the serum of patients with chronic progressive MS. Using gel chromatography, protein A sepharose afinity chromatography, and ELISAs for IL-2 and the IL-2 soluble receptor, we now demonstrate that this cytokine is bound to serum proteins. These serum proteins include antibodies to IL-2, soluble IL-2 receptors, and high-molecular-weight proteins. Using a CTLL cell assay, a serum fraction corresponding to IgG antibodies to IL-2 inhibited the activity of this cytokine. Thus, we present evidence far potential immunomodulation of a pivotal cytokine in MS by serum proteins.

AB - We previously reported elevations of interleukin 2 (IL-2) in the serum of patients with chronic progressive MS. Using gel chromatography, protein A sepharose afinity chromatography, and ELISAs for IL-2 and the IL-2 soluble receptor, we now demonstrate that this cytokine is bound to serum proteins. These serum proteins include antibodies to IL-2, soluble IL-2 receptors, and high-molecular-weight proteins. Using a CTLL cell assay, a serum fraction corresponding to IgG antibodies to IL-2 inhibited the activity of this cytokine. Thus, we present evidence far potential immunomodulation of a pivotal cytokine in MS by serum proteins.

UR - http://www.scopus.com/inward/record.url?scp=0029398646&partnerID=8YFLogxK

U2 - 10.1212/WNL.45.11.1971

DO - 10.1212/WNL.45.11.1971

M3 - Article

C2 - 7501143

AN - SCOPUS:0029398646

SN - 0028-3878

VL - 45

SP - 1971

EP - 1974

JO - Neurology

JF - Neurology

IS - 11

ER -

Interleukin-2 binding proteins in sera from normal subjects and multiple sclerosis patients

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