Interleukin-1 induces rapid and transient expression of the c-fos proto-oncogene in isolated pancreatic islets and in purified β-cells

Jonathan H. Hughes; Mark A. Watson; Richard A. Easom; John Turk; Michael L. McDaniel

doi:10.1016/0014-5793(90)81499-E

Interleukin-1 induces rapid and transient expression of the c-fos proto-oncogene in isolated pancreatic islets and in purified β-cells

Jonathan H. Hughes, Mark A. Watson, Richard A. Easom, John Turk, Michael L. McDaniel

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Abstract

The effect of interleukin-1β (IL-1) on expression of c-fos mRNA in isolated rat pancreatic islets was examined. Accumulation of c-fos mRNA was demonstrable after 30 min of exposure to IL-1, peaked by 60 min, and declined thereafter. Fluorescence-activated cell sorting (FACS) of dispersed islet cells was employed to localize the accumulation of c-fos mRNA to the β-cell. Cycloheximide did not influence the induction of c-fos mRNA by IL-1. Accumulation of c-fos mRNA therefore appears to be an early signal transduction event in the β-cell and a component of the cellular mechanism(s) by which IL-1 influences β-cell function.

Original language	English
Pages (from-to)	33-36
Number of pages	4
Journal	FEBS Letters
Volume	266
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)81499-E
State	Published - Jun 18 1990

Keywords

Insulin secretion
Interleukin 1
Pancreatic islet
c-fos
β-cell

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10.1016/0014-5793(90)81499-E

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@article{9b96364d26f34719a83395985554dac4,

title = "Interleukin-1 induces rapid and transient expression of the c-fos proto-oncogene in isolated pancreatic islets and in purified β-cells",

abstract = "The effect of interleukin-1β (IL-1) on expression of c-fos mRNA in isolated rat pancreatic islets was examined. Accumulation of c-fos mRNA was demonstrable after 30 min of exposure to IL-1, peaked by 60 min, and declined thereafter. Fluorescence-activated cell sorting (FACS) of dispersed islet cells was employed to localize the accumulation of c-fos mRNA to the β-cell. Cycloheximide did not influence the induction of c-fos mRNA by IL-1. Accumulation of c-fos mRNA therefore appears to be an early signal transduction event in the β-cell and a component of the cellular mechanism(s) by which IL-1 influences β-cell function.",

keywords = "Insulin secretion, Interleukin 1, Pancreatic islet, c-fos, β-cell",

author = "Hughes, {Jonathan H.} and Watson, {Mark A.} and Easom, {Richard A.} and John Turk and McDaniel, {Michael L.}",

note = "Funding Information: Acknowledgements: Fluorescence-activated cell sorting was performed by the Flow Cytometry Center at Washington University. The outstanding technical assistance of Joan Fink and Connie Marshall is greatly appreciated. This work was supported in part by a Medical Scientist Training Program Grant T32 GM07200 (J.H.H.) and National Institutes of Health Grants DK-34388, K04 DDK-01553 (J.T.), and DK-06181 (M.L.M.).",

year = "1990",

month = jun,

day = "18",

doi = "10.1016/0014-5793(90)81499-E",

language = "English",

volume = "266",

pages = "33--36",

journal = "FEBS Letters",

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T1 - Interleukin-1 induces rapid and transient expression of the c-fos proto-oncogene in isolated pancreatic islets and in purified β-cells

AU - Hughes, Jonathan H.

AU - Watson, Mark A.

AU - Easom, Richard A.

AU - Turk, John

AU - McDaniel, Michael L.

N1 - Funding Information: Acknowledgements: Fluorescence-activated cell sorting was performed by the Flow Cytometry Center at Washington University. The outstanding technical assistance of Joan Fink and Connie Marshall is greatly appreciated. This work was supported in part by a Medical Scientist Training Program Grant T32 GM07200 (J.H.H.) and National Institutes of Health Grants DK-34388, K04 DDK-01553 (J.T.), and DK-06181 (M.L.M.).

PY - 1990/6/18

Y1 - 1990/6/18

N2 - The effect of interleukin-1β (IL-1) on expression of c-fos mRNA in isolated rat pancreatic islets was examined. Accumulation of c-fos mRNA was demonstrable after 30 min of exposure to IL-1, peaked by 60 min, and declined thereafter. Fluorescence-activated cell sorting (FACS) of dispersed islet cells was employed to localize the accumulation of c-fos mRNA to the β-cell. Cycloheximide did not influence the induction of c-fos mRNA by IL-1. Accumulation of c-fos mRNA therefore appears to be an early signal transduction event in the β-cell and a component of the cellular mechanism(s) by which IL-1 influences β-cell function.

AB - The effect of interleukin-1β (IL-1) on expression of c-fos mRNA in isolated rat pancreatic islets was examined. Accumulation of c-fos mRNA was demonstrable after 30 min of exposure to IL-1, peaked by 60 min, and declined thereafter. Fluorescence-activated cell sorting (FACS) of dispersed islet cells was employed to localize the accumulation of c-fos mRNA to the β-cell. Cycloheximide did not influence the induction of c-fos mRNA by IL-1. Accumulation of c-fos mRNA therefore appears to be an early signal transduction event in the β-cell and a component of the cellular mechanism(s) by which IL-1 influences β-cell function.

KW - Insulin secretion

KW - Interleukin 1

KW - Pancreatic islet

KW - c-fos

KW - β-cell

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DO - 10.1016/0014-5793(90)81499-E

M3 - Article

C2 - 2114318

AN - SCOPUS:0025341797

SN - 0014-5793

VL - 266

SP - 33

EP - 36

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Interleukin-1 induces rapid and transient expression of the c-fos proto-oncogene in isolated pancreatic islets and in purified β-cells

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Keywords

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