Interferon-mediated induction of Ia antigen expression on isolated murine whole islets and dispersed islet cells

J. R. Wright, P. E. Lacy, E. R. Unanue, C. Muszynski, V. Hauptfeld

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32 Scopus citations

Abstract

Islets from male B10.BR mice (H-2(k)) were isolated by the collagenase technique, handpicked with a Pasteur pipette, and incubated (either intact or after dispersion with Dispase) for 0, 3, 5, 7, 10, or 14 days in tissue culture medium supplemented with either lymphokine supernatants or recombinant murine interferon-γ. Islets and single cells were examined for IA(k) molecules by use of indirect immunofluorescence. Ia-positive islet cells were identified on the surface of islets incubated with 5-10% lymphokine for >4 days or with 10, 100, or 1000 ng/ml interferon for >6 days. Islets incubated in unsupplemented medium were Ia negative. Incubation with 5% lymphokine induced Ia expression on 10-40% dispersed islet cultured for >9 days. Dual immunofluorescent staining for Ia and insulin revealed that Ia-positive cells included both β- and non-β-cells.

Original languageEnglish
Pages (from-to)1174-1177
Number of pages4
JournalDiabetes
Volume35
Issue number10
DOIs
StatePublished - 1986

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