Interface of biomolecular condensates modulates redox reactions

Yifan Dai, Christian F. Chamberlayne, Marco S. Messina, Christopher J. Chang, Richard N. Zare, Lingchong You, Ashutosh Chilkoti

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Biomolecular condensates mediate diverse cellular processes. The density-transition process of condensate formation results in the selective partitioning of molecules, which defines a distinct chemical environment within the condensates. However, the fundamental features of the chemical environment and the mechanisms by which such an environment can contribute to condensate functions have not been revealed. Here, we report that an electric potential gradient, thereby an electric field, is established at the liquid-liquid interface between the condensate and the bulk environment as a result of the density transition of ions and molecules brought about by phase separation. We find that the interface of condensates can drive spontaneous redox reactions in vitro and in living cells. Our results uncover a fundamental physicochemical property of the interface of condensates and the mechanism by which the interface can modulate biochemical activities.

Original languageEnglish
Pages (from-to)1594-1609
Number of pages16
JournalChem
Volume9
Issue number6
DOIs
StatePublished - Jun 8 2023

Keywords

  • biomolecular condensates
  • chemistry of condensate
  • interfacial electric field
  • liquid-liquid interface
  • reactive oxygen species
  • SDG3: Good health and well-being
  • spontaneous redox reaction

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