TY - JOUR
T1 - Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling
AU - Moe-Lange, Jacob
AU - Gappel, Nicoline M.
AU - Machado, Mackenzie
AU - Wudick, Michael M.
AU - Sies, Cosima S.A.
AU - Schott-Verdugo, Stephan N.
AU - Bonus, Michele
AU - Mishra, Swastik
AU - Hartwig, Thomas
AU - Bezrutczyk, Margaret
AU - Basu, Debarati
AU - Farmer, Edward E.
AU - Gohlke, Holger
AU - Malkovskiy, Andrey
AU - Haswell, Elizabeth S.
AU - Lercher, Martin J.
AU - Ehrhardt, David W.
AU - Frommer, Wolf B.
AU - Kleist, Thomas J.
N1 - Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved;
PY - 2021/9
Y1 - 2021/9
N2 - Glutamate has dual roles in metabolism and signaling; thus, signaling functions must be isolatable and distinct from metabolic fluctuations, as seen in low-glutamate domains at synapses. In plants, wounding triggers electrical and calcium (Ca2+) signaling, which involve homologs of mammalian glutamate receptors. The hydraulic dispersal and squeeze-cell hypotheses implicate pressure as a key component of systemic signaling. Here, we identify the stretch-activated anion channel MSL10 as necessary for proper wound-induced electrical and Ca2+ signaling. Wound gene induction, genetics, and Ca2+ imaging indicate that MSL10 acts in the same pathway as the glutamate receptor–like proteins (GLRs). Analogous to mammalian NMDA glutamate receptors, GLRs may serve as coincidence detectors gated by the combined requirement for ligand binding and membrane depolarization, here mediated by stretch activation of MSL10. This study provides a molecular genetic basis for a role of mechanical signal perception and the transmission of long-distance electrical and Ca2+ signals in plants.
AB - Glutamate has dual roles in metabolism and signaling; thus, signaling functions must be isolatable and distinct from metabolic fluctuations, as seen in low-glutamate domains at synapses. In plants, wounding triggers electrical and calcium (Ca2+) signaling, which involve homologs of mammalian glutamate receptors. The hydraulic dispersal and squeeze-cell hypotheses implicate pressure as a key component of systemic signaling. Here, we identify the stretch-activated anion channel MSL10 as necessary for proper wound-induced electrical and Ca2+ signaling. Wound gene induction, genetics, and Ca2+ imaging indicate that MSL10 acts in the same pathway as the glutamate receptor–like proteins (GLRs). Analogous to mammalian NMDA glutamate receptors, GLRs may serve as coincidence detectors gated by the combined requirement for ligand binding and membrane depolarization, here mediated by stretch activation of MSL10. This study provides a molecular genetic basis for a role of mechanical signal perception and the transmission of long-distance electrical and Ca2+ signals in plants.
UR - http://www.scopus.com/inward/record.url?scp=85115240520&partnerID=8YFLogxK
U2 - 10.1126/sciadv.abg4298
DO - 10.1126/sciadv.abg4298
M3 - Article
C2 - 34516872
AN - SCOPUS:85115240520
SN - 2375-2548
VL - 7
JO - Science Advances
JF - Science Advances
IS - 37
M1 - eabg4298
ER -