TY - JOUR
T1 - Interaction of murine colony-stimulating factor (CSF-1) with alveolar mononuclear phagocytes
AU - Chen, Dien Ming
AU - Lin, Hsiu San
N1 - Funding Information:
The authors wish to thank mr G. Satkowski for his technical assistance and Josie Garcia for typing the manuscript.W e are grateful to dr Charles Kuhn, III for his valuable suggestions and discussions. This work was supported by grants HL-19746 from the National Heart, Lung and Blood Institute and AI-15542f rom the National Institute of Allergy and In-fectiousD iseases.
PY - 1982/8
Y1 - 1982/8
N2 - Colony-stimulating factor (CSF-1) was purified from serum-free L-cell-conditioned medium (LCM) and iodinated so that we could study its interaction with murine alveolar macrophages. At 0 °C, the binding of 125ICSF-1 to alveolar macrophages reached a stable maximum within 16 h. Under this condition, the binding of 125ICSF-1 at various concentrations was saturated at about 3 ng/ml. The binding sites of 125ICSF-1 were sensitive to trypsin but not to DNase or RNase treatment. At 37 °C, the trypsin-treated cells regenerated more than 90% of their original binding sites within 12 h. Whereas more than 97% of these alveolar macrophages were phagocytic and esterase-positive, autoradiographic studies showed that only 10-31 % of them were capable of binding to 125ICSF-1. These results indicate that the frequencies of CSF-1-binding cells and alveolar macrophage colony-forming cells (AL-CFC) are closely correlated, but no causal relationship has been established.
AB - Colony-stimulating factor (CSF-1) was purified from serum-free L-cell-conditioned medium (LCM) and iodinated so that we could study its interaction with murine alveolar macrophages. At 0 °C, the binding of 125ICSF-1 to alveolar macrophages reached a stable maximum within 16 h. Under this condition, the binding of 125ICSF-1 at various concentrations was saturated at about 3 ng/ml. The binding sites of 125ICSF-1 were sensitive to trypsin but not to DNase or RNase treatment. At 37 °C, the trypsin-treated cells regenerated more than 90% of their original binding sites within 12 h. Whereas more than 97% of these alveolar macrophages were phagocytic and esterase-positive, autoradiographic studies showed that only 10-31 % of them were capable of binding to 125ICSF-1. These results indicate that the frequencies of CSF-1-binding cells and alveolar macrophage colony-forming cells (AL-CFC) are closely correlated, but no causal relationship has been established.
UR - http://www.scopus.com/inward/record.url?scp=0020447618&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(82)90121-5
DO - 10.1016/0014-4827(82)90121-5
M3 - Article
C2 - 6981518
AN - SCOPUS:0020447618
SN - 0014-4827
VL - 140
SP - 323
EP - 329
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -