Interaction of human immunodeficiency virus type 2 Vpx and invariant chain

Heather A. Pancio, Nancy Vander Heyden, Kavitha Kosuri, Peter Cresswell, Lee Ratner

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Vpx is a virion-associated protein of human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency viruses. The yeast two-hybrid system was used to identify invariant chain (Ii) as a cellular protein that interacts with HIV-2 Vpx. Vpx-Ii interaction was confirmed in cell-free reactions using bacterially expressed glutathione S-transferase fusion proteins and by coimmunoprecipitation in transfected and infected cells. In chronically infected cells expressing Vpx, Ii levels were markedly decreased, presumably due to enhanced degradation. These findings suggest that Vpx may disrupt major histocompatibility complex class II antigen presentation.

Original languageEnglish
Pages (from-to)6168-6172
Number of pages5
JournalJournal of virology
Volume74
Issue number13
DOIs
StatePublished - 2000

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