Interaction and regulation between lipid mediator phosphatidic acid and circadian clock regulators

Sang Chul Kim, Dmitri A. Nusinow, Maria L. Sorkin, Jose Pruneda-Paz, Xuemin Wanga

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Circadian clocks play important roles in regulating cellular metabolism, but the reciprocal effect that metabolism has on the clock is largely unknown in plants. Here, we show that the central glycerolipid metabolite and lipid mediator phosphatidic acid (PA) interacts with and modulates the function of the core clock regulators LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) in Arabidopsis (Arabidopsis thaliana). PA reduced the ability of LHY and CCA1 to bind the promoter of their target gene TIMING OF CAB EXPRESSION1. Increased PA accumulation and inhibition of PAproducing enzymes had opposite effects on circadian clock outputs. Diurnal change in levels of several membrane phospholipid species, including PA, observed in wild type was lost in the LHY and CCA1 double knockout mutant. Storage lipid accumulation was also affected in the clock mutants. These results indicate that the interaction of PA with the clock regulator may function as a cellular conduit to integrate the circadian clock with lipid metabolism.

Original languageEnglish
Pages (from-to)399-416
Number of pages18
JournalPlant Cell
Issue number2
StatePublished - Feb 2019


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