Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis

Denise Stenzel, Andrea Lundkvist, Dominique Sauvaget, Marta Busse, Mariona Graupera, Arjan van der Flier, Errol S. Wijelath, Jacqueline Murray, Michael Sobel, Mercedes Costell, Seiichiro Takahashi, Reinhard Fässler, Yu Yamaguchi, David H. Gutmann, Richard O. Hynes, Holger Gerhardt

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Abstract

Fibronectin (FN) is a major component of the extracellular matrix and functions in cell adhesion, cell spreading and cell migration. In the retina, FN is transiently expressed and assembled on astrocytes (ACs), which guide sprouting tip cells and deposit a provisional matrix for sprouting angiogenesis. The precise function of FN in retinal angiogenesis is largely unknown. Using genetic tools, we show that astrocytes are the major source of cellular FN during angiogenesis in the mouse retina. Deletion of astrocytic FN reduces radial endothelial migration during vascular plexus formation in a gene dose-dependent manner. This effect correlates with reduced VEGF receptor 2 and PI3K/AKT signalling, and can be mimicked by selectively inhibiting VEGF-A binding to FN through intraocular injection of blocking peptides. By contrast, AC-specific replacement of the integrin-binding RGD sequence with FN-RGE or endothelial deletion of itga5 shows little effect on migration and PI3K/AKT signalling, but impairs filopodial alignment along AC processes, suggesting that FN-integrin α5β1 interaction is involved in filopodial adhesion to the astrocytic matrix. AC FN shares its VEGF-binding function and cell-surface distribution with heparan-sulfate (HS), and genetic deletion of both FN and HS together greatly enhances the migration defect, indicating a synergistic function of FN and HS in VEGF binding. We propose that in vivo the VEGF-binding properties of FN and HS promote directional tip cell migration, whereas FN integrin-binding functions to support filopodia adhesion to the astrocytic migration template.

Original languageEnglish
Pages (from-to)4451-4463
Number of pages13
JournalDevelopment
Volume138
Issue number20
DOIs
StatePublished - Oct 15 2011

Keywords

  • Angiogenesis
  • Cell migration
  • Endothelial guidance
  • Extracellular matrix
  • Tip cells

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    Stenzel, D., Lundkvist, A., Sauvaget, D., Busse, M., Graupera, M., van der Flier, A., Wijelath, E. S., Murray, J., Sobel, M., Costell, M., Takahashi, S., Fässler, R., Yamaguchi, Y., Gutmann, D. H., Hynes, R. O., & Gerhardt, H. (2011). Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis. Development, 138(20), 4451-4463. https://doi.org/10.1242/dev.071381