TY - JOUR
T1 - Integrated photoacoustic, confocal, and two-photon microscope
AU - Rao, Bin
AU - Soto, Florentina
AU - Kerschensteiner, Daniel
AU - Wang, Lihong V.
N1 - Funding Information:
The authors thank Ms. DeGenova Sarah for her assistance in preparation of zebrafish samples and Professor James Ballard for his technical writing support. We thank Dr. Lijun Ma and Dr. Yu Wang for their help on the LabVIEW program. Institutional support from the Nano Research Facility of Washington University in St. Louis is appreciated. This work was sponsored in part by National Institutes of Health (NIH) grants 1S10RR028864, K99AR062530, DP1 EB016986 (NIH Director’s Pioneer Award), and R01 CA159959. L. V. Wang has financial interests in Microphotoacoustics Inc. and Endra Inc., which did not support this work.
PY - 2014/3
Y1 - 2014/3
N2 - The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries.
AB - The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries.
KW - Confocal microscopy
KW - Optical-resolution photoacoustic microscopy
KW - Photoacoustic microscopy
KW - Two-photon microscopy
UR - http://www.scopus.com/inward/record.url?scp=84897772689&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.19.3.036002
DO - 10.1117/1.JBO.19.3.036002
M3 - Article
C2 - 24589986
AN - SCOPUS:84897772689
SN - 1083-3668
VL - 19
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 3
M1 - 036002
ER -