TY - JOUR
T1 - Insulin increases expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rat hepatocytes
AU - Von Wronski, Mathew A.
AU - Hirano, Ken Ichi
AU - Cagen, Lauren M.
AU - Wilcox, Henry G.
AU - Raghow, Rajendra
AU - Thorngate, Fayanne E.
AU - Heimberg, Murray
AU - Davidson, Nicholas O.
AU - Elam, Marshall B.
N1 - Funding Information:
From the Departments of Pharmacology and Medicine, and Division of Clinical Pharmacology, The Health Science Center, University of Tennessee, Memphis, TN; Department of Pharmacological Sciences, State University of New York, Health Science Center, Stony Brook, NY," Department of Medicine, University of Chicago, Chicago, Chicago, IL; and the Memphis Veterans Administration Medical Center, Memphis, TN. Submitted September 23, 1997; accepted January 7, 1998. Supported by a Merit Review Grant from the Department of Veterans Affairs (to M.B.E.), by US Public Health Service Grant No. GR-HL-07641-09 (to M.A.V.), and by HL-38180, HL-18577, and DK-42086 (to N.O.D.). Address reprint requests to Marshall B. Elam, PhD, MD, Department of Pharmacology, University of Tennessee Health Sciences Center, 874 Union Ave, Memphis, TN38163. Copyright © 1998 by W.B. Saunders Company 0026-0495/98/4707-0019503.00/0
PY - 1998
Y1 - 1998
N2 - We have previously shown that chronic insulin treatment of rat hepatocytes increases the fraction of edited apolipoprotein B (apoB) mRNA from approximately 50% to as much as 90%. We have now examined the effect of insulin on apobec-1 mRNA abundance and demonstrate that increased editing of apoB mRNA following insulin treatment is accompanied by elevated apobec-1 mRNA levels in primary rat hepatocytes. Time-course measurements of the effects of insulin on apoB mRNA editing and apobec-1 mRNA abundance showed that both were elevated almost maximally within 48 hours and sustained for at least 5 days of insulin treatment.
AB - We have previously shown that chronic insulin treatment of rat hepatocytes increases the fraction of edited apolipoprotein B (apoB) mRNA from approximately 50% to as much as 90%. We have now examined the effect of insulin on apobec-1 mRNA abundance and demonstrate that increased editing of apoB mRNA following insulin treatment is accompanied by elevated apobec-1 mRNA levels in primary rat hepatocytes. Time-course measurements of the effects of insulin on apoB mRNA editing and apobec-1 mRNA abundance showed that both were elevated almost maximally within 48 hours and sustained for at least 5 days of insulin treatment.
UR - http://www.scopus.com/inward/record.url?scp=0031837340&partnerID=8YFLogxK
U2 - 10.1016/S0026-0495(98)90128-7
DO - 10.1016/S0026-0495(98)90128-7
M3 - Article
C2 - 9667237
AN - SCOPUS:0031837340
SN - 0026-0495
VL - 47
SP - 869
EP - 873
JO - Metabolism: clinical and experimental
JF - Metabolism: clinical and experimental
IS - 7
ER -