Insights of the tubulin code in gametes and embryos: From basic research to potential clinical applications in humans

Farners Amargant, Montserrat Barragan, Rita Vassena, Isabelle Vernos

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations

Abstract

Microtubules are intracellular filaments that define in space and in time a large number of essential cellular functions such as cell division, morphology and motility, intracellular transport and flagella and cilia assembly. They are therefore essential for spermatozoon and oocyte maturation and function, and for embryo development. The dynamic and functional properties of the microtubules are in large part defined by various classes of interacting proteins including MAPs (microtubule associated proteins), microtubule-dependent motors, and severing and modifying enzymes. Multiple mechanisms regulate these interactions. One of them is defined by the high diversity of the microtubules themselves generated by the combination of different tubulin isotypes and by several tubulin post-translational modifications (PTMs). This generates a so-called tubulin code that finely regulates the specific set of proteins that associates with a given microtubule thereby defining the properties and functions of the network. Here we provide an in depth review of the current knowledge on the tubulin isotypes and PTMs in spermatozoa, oocytes, and preimplantation embryos in various model systems and in the human species. We focus on functional implications of the tubulin code for cytoskeletal function, particularly in the field of human reproduction and development, with special emphasis on gamete quality and infertility. Finally, we discuss some of the knowledge gaps and propose future research directions.

Original languageEnglish
Pages (from-to)575-589
Number of pages15
JournalBiology of reproduction
Volume100
Issue number3
DOIs
StatePublished - Mar 1 2019

Keywords

  • Early embryo development
  • Oocytes
  • Spermatozoa
  • Tubulin
  • Tubulin isotypes
  • Tubulin post-translational modifications (PTMs)

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