Insights into the NOX NADPH oxidases using heterologous whole cell assays

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Assays based on ectopic expression of NOX NADPH oxidase subunits in heterologous mammalian cells are an important approach for investigating features of this family of enzymes. These model systems have been used to analyze the biosynthesis and functional domains of NOX enzyme components as well as their regulation and cellular activities. This chapter provides an overview of the basic principles and applications of heterologous whole cell assays in studying NOX NADPH oxidases.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-151
Number of pages13
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1982
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • COS-7
  • Expression
  • Flavocytochrome b
  • NOX
  • phox

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    Dinauer, M. C. (2019). Insights into the NOX NADPH oxidases using heterologous whole cell assays. In Methods in Molecular Biology (pp. 139-151). (Methods in Molecular Biology; Vol. 1982). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9424-3_9