Assays based on ectopic expression of NOX NADPH oxidase subunits in heterologous mammalian cells are an important approach for investigating features of this family of enzymes. These model systems have been used to analyze the biosynthesis and functional domains of NOX enzyme components as well as their regulation and cellular activities. This chapter provides an overview of the basic principles and applications of heterologous whole cell assays in studying NOX NADPH oxidases.
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||13|
|State||Published - 2019|
|Name||Methods in Molecular Biology|
- Flavocytochrome b