Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping

Carmelle T. Norice-Tra; José Ribeiro; Sasisekhar Bennuru; Michael P. Fay; Rahul Tyagi; Makedonka Mitreva; Thomas B. Nutman

doi:10.1093/infdis/jix394

Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping

Carmelle T. Norice-Tra
, José Ribeiro
, Sasisekhar Bennuru
, Michael P. Fay
, Rahul Tyagi
, Makedonka Mitreva
, Thomas B. Nutman

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.

Original language	English
Pages (from-to)	736-743
Number of pages	8
Journal	Journal of Infectious Diseases
Volume	216
Issue number	6
DOIs	https://doi.org/10.1093/infdis/jix394
State	Published - Sep 15 2017

Keywords

Onchocerca volvulus
immunophenotype
immunoreactivity
population biology
single nucleotide polymorphism

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10.1093/infdis/jix394

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@article{81ca4e3afd7e4912807136065f55e938,

title = "Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping",

abstract = "We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.",

keywords = "Onchocerca volvulus, immunophenotype, immunoreactivity, population biology, single nucleotide polymorphism",

author = "Norice-Tra, \{Carmelle T.\} and Jos{\'e} Ribeiro and Sasisekhar Bennuru and Fay, \{Michael P.\} and Rahul Tyagi and Makedonka Mitreva and Nutman, \{Thomas B.\}",

note = "Publisher Copyright: {\textcopyright} 2017 The Author.",

year = "2017",

month = sep,

day = "15",

doi = "10.1093/infdis/jix394",

language = "English",

volume = "216",

pages = "736--743",

journal = "Journal of Infectious Diseases",

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TY - JOUR

T1 - Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping

AU - Norice-Tra, Carmelle T.

AU - Ribeiro, José

AU - Bennuru, Sasisekhar

AU - Fay, Michael P.

AU - Tyagi, Rahul

AU - Mitreva, Makedonka

AU - Nutman, Thomas B.

PY - 2017/9/15

Y1 - 2017/9/15

N2 - We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.

AB - We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.

KW - Onchocerca volvulus

KW - immunophenotype

KW - immunoreactivity

KW - population biology

KW - single nucleotide polymorphism

UR - https://www.scopus.com/pages/publications/85030621825

U2 - 10.1093/infdis/jix394

DO - 10.1093/infdis/jix394

M3 - Article

C2 - 28934436

AN - SCOPUS:85030621825

SN - 0022-1899

VL - 216

SP - 736

EP - 743

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 6

ER -

Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping

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