Injectable in situ Physically and Chemically Crosslinkable Gellan Hydrogel

Hongwei Du, Paul Hamilton, Mattew Reilly, Nathan Ravi

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39 Scopus citations


An injectable, in situ physically and chemically crosslinkable gellan hydrogel is synthesized via gellan thiolation. The thiolation does not alter the gellan's unique 3-D conformation, but leads to a lower phase transition temperature under physiological conditions and stable chemical crosslinking. The synthesis and hydrogels are characterized by 1H NMR, FT-IR, CD, or rheology measurements. The injectability and the tissue culture cell viability is also tested. The thiolated gellan hydrogel exhibits merits, such as ease for injection, quick gelation, lower gelling temperature, stable structure, and nontoxicity, which make it promising in biomedicine and bioengineering as an injectable hydrogel. The introduction of the small molecule cysteine into gellan leads to a hydrogel system with reduced gelling temperature and stable chemical crosslinks. The as-prepared hydrogel is an entity of a physical hydrogel and a chemical hydrogel, possessing merits of both, which makes it desirable for a new injectable hydrogel.

Original languageEnglish
Pages (from-to)952-961
Number of pages10
JournalMacromolecular Bioscience
Issue number7
StatePublished - Jul 2012


  • Biomaterials
  • Double helix
  • Gellan
  • Hydrogel
  • Injectable


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