Initiation of meiotic development is controlled by three post-transcriptional pathways in caenorhabditis elegans

Ariz Mohammad, Kara Vanden Broek, Christopher Wang, Anahita Daryabeigi, Verena Jantsch, Dave Hansen, Tim Schedl

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A major event in germline development is the transition from stem/progenitor cells to entry into meiosis and gametogenesis. This transition requires downregulation of mitotic cell cycle activity and upregulation of processes associated with meiosis. We identify the Caenorhabditis elegans SCFPROM-1 E3 ubiquitin-ligase complex as functioning to downregulate mitotic cell cycle protein levels including cyclin E, WAPL-1, and KNL-2 at meiotic entry and, independently, promoting homologous chromosome pairing as a positive regulator of the CHK-2 kinase. SCFPROM-1 is thus a novel regulator of meiotic entry, coordinating downregulation of mitotic cell cycle proteins and promoting homolog pairing. We further show that SCFPROM-1 functions redundantly, in parallel to the previously described GLD-1 and GLD-2 meiotic entry pathways, downstream of and inhibited by GLP-1 Notch signaling, which specifies the stem cell fate. Accordingly, C. elegans employs three post-transcriptional pathways, SCFPROM-1-mediated protein degradation, GLD-1-mediated translational repression, and GLD-2-mediated translational activation, to control and coordinate the initiation of meiotic development.

Original languageEnglish
Pages (from-to)1197-1224
Number of pages28
JournalGenetics
Volume209
Issue number4
DOIs
StatePublished - Aug 2018

Keywords

  • GLD-1
  • GLD-2
  • Germline
  • Meiotic development
  • Meiotic entry
  • PROM-1
  • SCF

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