Inhibition of PPAR-α activity in mice with cardiac-restricted expression of tumor necrosis factor: Potential role of TGF-β/Smad3

Kenichi Sekiguchi, Qi Tian, Masakuni Ishiyama, Jana Burchfield, Feng Gao, Douglas L. Mann, Philip M. Barger

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

A shift in energy substrate utilization from fatty acids to glucose has been reported in failing hearts, resulting in improved oxygen efficiency yet perhaps also contributing to a state of energy deficiency. Peroxisome proliferator-activated receptor (PPAR)-α, the principal transcriptional regulator of cardiac fatty acid β-oxidation (FAO) genes, is downregulated in heart failure, and this may contribute to reduced fatty acid utilization. Cardiomyopathic states are also accompanied by elevated levels of circulating cytokines, such as tumor necrosis factor (TNF), as well as increased local production of cytokines and profibrotic factors, such as transforming growth factor (TGF)-β. However, whether these molecular pathways directly modulate cardiac energy metabolism and PPAR-α activity is not known. Therefore, FAO capacity and FAO gene expression were determined in mice with cardiac-restricted overexpression of TNF (MHCsTNF3). MHCsTNF 3 hearts had significantly lower FAO capacity and decreased expression of PPAR-α and FAO target genes compared with control hearts. Surprisingly, TNF had little effect on PPAR-α activity and FAO rates in cultured ventricular myocytes, suggesting that TNF acts indirectly on myocyte FAO in vivo. We found that TGF-β expression was upregulated in MHCsTNF 3 hearts and that treatment of cultured myocytes with TGF-β significantly suppressed FAO rates and directly impaired PPAR-α activity, a result reproduced by Smad3 overexpression. This work demonstrates that TGF-β signaling pathways directly suppress PPAR-α activity and reduce FAO in cardiac myocytes, perhaps in response to locally elevated TNF. Although speculative, TGF-β-driven repair mechanisms may also include the additional benefit of limiting FAO in injured myocardium.

Original languageEnglish
Pages (from-to)H1443-H1451
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume292
Issue number3
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

Keywords

  • Genes
  • Inflammation
  • Metabolism
  • Signal transduction

Fingerprint Dive into the research topics of 'Inhibition of PPAR-α activity in mice with cardiac-restricted expression of tumor necrosis factor: Potential role of TGF-β/Smad3'. Together they form a unique fingerprint.

Cite this