@article{b6b9482fdecb42adbee5ef0a9e97e1e2,
title = "Inhibition of lymphocyte activation by inhibitors of γ-glutamyl transpeptidase",
author = "Wedner, {H. J.}",
note = "Funding Information: Inability to develop CTLs in vivo to hapten altered self can be attributed in part to an inhibitor of interleukin 2 (IL-2) which is present in the serum of normal mice. We have shown earlier that hapten specific CTLs can be generated in C3H mice (H-2 k, Mls c) provided CBA/J (H-2 k Mls d) spleen cells are injected simultaneously with hapten-modified syngeneic spleen cells into the hind foot paws. Using a murine IL-2 dependent, cloned cytotoxic T cell line, CT-6, we have compared the activity of inhibitor of IL-2 in serum, at intervals, following the injection of syngeneic spleen cells, CBA spleen cells, TNP-C3H spleen cells alone or together with CBA spleen cells. The results indicate that inhibitor was neutralized optimally 48-72 hrs. following injection of TNP-C3H spleen cells mixed with CBA/J spleen cells. The order in which neutralization occurred was as follows: TNP-C3H cells + CBA/J cells > CBA cells >TNP-C3H cells > normal C3H spleen cells. Furthermore, supernatants from cultures of C3H lymph node cells stimulated in vivo with CBA/J cells also contained IL-2 activity. Thus, injection of CBA/J cells with TNP-modified syngeneic spleen cells produces IL-2 in vivo in sufficient quantity to neutralize the activity of the inhibitor as well as to facilitate the maturation of pre-CTLs into hapten-altered self specific CTLs. (Supported in part by USPHS NIH Grant AI-17657).",
year = "1982",
doi = "10.1016/0192-0561(82)90382-4",
language = "English",
volume = "4",
pages = "363",
journal = "International Journal of Immunopharmacology",
issn = "0192-0561",
number = "4",
}