Inhibition of lipases by ε-polylysine

Takahiro Tsujita, Maho Sumiyoshi, Takeshi Takaku, William E. Momsen, Mark E. Lowe, Howard L. Brockman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Oral administration of ε-polylysine to rats reduced the peak plasma triacylglycerol concentration. In vitro, ε-polylysine and polylysine strongly inhibited the hydrolysis, by either pancreatic lipase or carboxylester lipase, of trioleoylglycerol (TO) emulsified with phosphatidylcholine (PC) and taurocholate. The ε-polylysine concentration required for complete inhibition of pancreatic lipase, 10 μg/ml, is 1,000 times lower than that of BSA required for the same effect. Inhibition requires the presence of bile salt and, unlike inhibition of lipase by other proteins, is not reversed by supramicellar concentrations of bile salt. Inhibition increases with the degree of polylysine polymerization, is independent of lipase concentration, is independent of pH between 5.0 and 9.5, and is accompanied by an inhibition of lipase binding to TO-PC emulsion particles. However, ε-polylysine did not inhibit the hydrolysis by pancreatic lipase of TO emulsions prepared using anionic surfactants, TO hydrolysis catalyzed by lingual lipase, or the hydrolysis of a water-soluble substrate. In the presence of taurocholate, ε-polylysine becomes surface active and adsorbs to TO-PC monomolecular films. These results are consistent with ε-polylysine and taurocholate forming a surface-active complex that binds to emulsion particles, thereby retarding lipase adsorption and triacylglycerol hydrolysis both in vivo and in vitro.

Original languageEnglish
Pages (from-to)2278-2286
Number of pages9
JournalJournal of lipid research
Volume44
Issue number12
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Basic peptide
  • Complex
  • Lipid emulsion
  • Lipid monolayer

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    Tsujita, T., Sumiyoshi, M., Takaku, T., Momsen, W. E., Lowe, M. E., & Brockman, H. L. (2003). Inhibition of lipases by ε-polylysine. Journal of lipid research, 44(12), 2278-2286. https://doi.org/10.1194/jlr.M300151-JLR200